Unit:
Speciality PeriodontologyΒιβλιοθήκη Οδοντιατρικής
Supervisors info:
Πεπελάση Ευδοξία, Καθηγήτρια, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Ιωάννης Καρούσης, Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Γεώργιος Μπομπέτσης, Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Original Title:
The effect of photobiomodulation with an 810 nm diode laser on human gingival fibroblasts cultured on biofilm coated and air-flow decontaminated smooth titanium discs. In vitro study
Translated title:
The effect of photobiomodulation with an 810 nm diode laser on human gingival fibroblasts cultured on biofilm coated and air-flow decontaminated smooth titanium discs. In vitro study
Summary:
Introduction: In terms of the treatment for peri-implant mucositis, the ultimate therapeutic goal is to remove the microbial biofilm from the implant surface as well as to preserve a titanium surface that promotes the re-establishment of the soft tissue seal. The cleaning efficacy of the air-flow decontamination (with erythritol powder) is well documented, though there are no data regarding the biocompatibility of the air-flow decontaminated smooth titanium surface. Moreover, a positive upgrowth stimulatory effect of Low Level Laser Therapy (LLLT) on gingival fibroblasts cultured on air-flow decontaminated smooth titanium surfaces might have clinical applications in the re-establishment of the implant-soft tissue interface by enhancing the healing process.
Objective: The aim of the present in vitro study was to evaluate the effect of air-flow decontamination (with erythritol powder) on the proliferation and growth factor expression of human gingival fibroblasts (HGFs) cultured on smooth titanium discs that had been coated with biofilm as well as the effect of photobiomodulation with an 810 nm diode laser on the proliferation and growth factor expression of HGFs cultured on smooth titanium discs that had been coated with biofilm and decontaminated with the application of air-flow by using erythritol powder.
Materials and methods: A total of 240 smooth titanium discs (PT, Strauman®) were studied. Peri-implant mucositis conditions were simulated by contaminating 120 discs with 4 bacteria strains. Six groups of HGFs were studied: HGFs cultured on 60 sterile discs (group I), HGFs cultured on 60 discs coated with biofilm and decontaminated with air-flow (with erythritol powder) (group II), HGFs cultured on 60 sterile discs and irradiated with an 810 nm diode laser (15 J/cm2, 500 mW) (group III), HGFs cultured on 60 biofilm-coated and air-flow decontaminated (with erythritol powder) discs and irradiated with an 810 nm diode laser (group IV), HGFs cultured on tissue culture plates (TCP) and irradiated with an 810 nm diode laser (group V) and HGFs cultured on TCP (control group). Cell viability was evaluated at 24, 48 and 72 hours by MTT assay and FDA/PI staining. Gene expression of epidermal growth factor (EGF), transforming growth factor-beta1 (TGF-β1), fibroblast growth factor (FGF), collagen type I alpha 1 chain (COL1A1) and matrix metalloproteinase 1 (MMP1) was evaluated at 7, 14, 21 days by RT-PCR. SEM microscopy was performed at 7, 14, 21 days for all disc groups.
Results: Cell proliferation presented statistically significant increase between 24 and 72 hours for all groups, except for group I. At 14 days, group II presented statistically significantly higher proliferation as compared with groups I and IV. At 72 hours, group I showed statistically significantly lower cell proliferation as compared with all other groups. For EGF expression, groups II and III had statistically significantly higher expression as compared with group I at 14 days. COL1A1 gene expression was statistically significantly higher for group IV as compared with group I at 7 days, as well as for group IV as compared with groups I and II at 14 days. For MMP1 expression, group II presented statistically significantly lower expression as compared witrh all other groups at 14 days.
Conclusions: Within its limits, this in vitro study demonstrated that for HGFs cultured on smooth titanium discs, cell proliferation was significantly increased by photobiomodulation of the cells with an 810 nm diode laser, erythritol-based air-flow decontamination of the disc and their combination. Gene expression at 14 days of culture was significantly increased for EGF by erythritol-based air-flow decontamination and by photobiomodulation with an 810 nm diode laser as well as for COL1A1 by their combination. The addition of cell photobiomodulation with an 810 nm diode laser to air-flow disc decontamination significantly increased COL1A1 and MMP1 gene expression at 14 days of culture.
Clinical Relevance: The present results indicate that the erythritol-based air-flow decontamination of the smooth titanium surface and the subsequent photobiomodulation of the soft peri-implant tissues with an 810 nm diode laser might prove to be of great importance for the treatment of peri-implant mucositis aiming to enhance soft tissue adhesion to titanium implant abutments.
Main subject category:
Health Sciences
Keywords:
Photobiomodulation, Peri-implant mucositis, Smooth titanium surface, Gingival fibroblasts, Air-flow
Number of references:
427
File:
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Sykara_Maria_Master.pdf
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File access is restricted until 2025-06-09.
