Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells

Επιστημονική δημοσίευση - Άρθρο Περιοδικού
uoadl:3000363

Μονάδες

Ερευνητικό υλικό ΕΚΠΑ

Τίτλος

Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells

Γλώσσες Τεκμηρίου

Αγγλικά

Περίληψη

Human mononuclear phagocytes comprise phenotypically and functionally overlapping subsets of dendritic cells (DCs) and monocytes, but the extent of their heterogeneity and distinct markers for subset identification remains elusive. By integrating high-dimensional single-cell protein and RNA expression data, we identified distinct markers to delineate monocytes from conventional DC2 (cDC2s). Using CD88 and CD89 for monocytes and HLA-DQ and FcεRIα for cDC2s allowed for their specific identification in blood and tissues. We also showed that cDC2s could be subdivided into phenotypically and functionally distinct subsets based on CD5, CD163, and CD14 expression, including a distinct subset of circulating inflammatory CD5−CD163+CD14+ cells related to previously defined DC3s. These inflammatory DC3s were expanded in systemic lupus erythematosus patients and correlated with disease activity. These findings further unravel the heterogeneity of DC subpopulations in health and disease and may pave the way for the identification of specific DC subset-targeting therapies. Using high-dimensional protein and RNA single-cell analyses, Dutertre et al. analyze human dendritic cell and monocyte subsets and identify markers that delineate them and unravel their heterogeneity. They also reveal the presence of inflammatory CD14+ DC3s, a subset of cDC2s, that correlate with disease progression and may be functionally involved in systemic lupus erythematosus immunopathology. © 2019 Elsevier Inc.

Έτος δημοσίευσης

2019

Συγγραφείς

Dutertre, C.-A. Becht, E. Irac, S.E. Khalilnezhad, A. Narang, V. Khalilnezhad, S. Ng, P.Y. van den Hoogen, L.L. Leong, J.Y. Lee, B. Chevrier, M. Zhang, X.M. Yong, P.J.A. Koh, G. Lum, J. Howland, S.W. Mok, E. Chen, J. Larbi,...

Περισσότερα

Περιοδικό

Pathogens and Immunity

Εκδότης

null

Τόμος

51

Αριθμός / τεύχος

3

Σελίδες

573-589.e8

Τελευταία τροποποίηση

πριν από 3 έτη

Άδεια χρήσης

Creative Commons Αναφορά Δημιουργού-Μη Εμπορική Χρήση 4.0 (CC-BY-NC)

Εξαγωγή Citation

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