Τίτλος:
Concordance of genotypic resistance interpretation algorithms in HIV-1 infected patients: An exploratory analysis in Greece
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Purpose: Genotypic resistance-related mutations in HIV-1 disease are often difficult to interpret. Different algorithms have been developed to provide meaningful application into clinical context. We aimed to compare, for the first time in Greece, the results of genotypic resistance derived from three interpretation algorithms. Methods: The sequences of 120 HIV 1-infected patients were tested for genotypic resistance to 19 antiretroviral (ARV) drugs (n = 2280 sequences). The interpretation results of Rega, ANRS and ViroSeq algorithms were compared. Results: Complete concordance was found for 2/19 ARV drugs, namely lamivudine and emptricitabine. Concordance was high for nucleoside reverse transcriptase inhibitors (NRTIs) and low for protease inhibitors (PIs). In inter-algorithm pairs, agreement was high between Rega and ViroSeq (kappa = 0.701), especially by ARV class, namely NRTIs (k = 0.869) and NNRTIs (k = 0.562). The only exception was noted for rilpivirine, where agreement was higher between ANRS and Rega (k = 0.410) compared to other inter-algorithm pairs (k = 0.018−0.055). By contrast, for PIs all comparisons yielded concordance equivalent to chance (k = 0.000). Conclusions: Our exploratory analysis provided evidence of significant inter-algorithm discordances, especially for PIs and NNRTIs highlighting the importance of matching the results of different algorithms to achieve optimized risk stratification. Ongoing research could assist clinical physicians in interpreting complex genotypic resistance patterns. © 2021 Elsevier B.V.
Συγγραφείς:
Kantzanou, M.
Karalexi, M.A.
Zivinaki, A.
Riza, E.
Papachristou, H.
Vasilakis, A.
Kontogiorgis, C.
Linos, A.
Περιοδικό:
Journal of Clinical Virology
Λέξεις-κλειδιά:
abacavir; amprenavir; atazanavir; darunavir; didanosine; efavirenz; emtricitabine; etravirine; indinavir; lamivudine; lopinavir plus ritonavir; nevirapine; rilpivirine; stavudine; tenofovir; zidovudine; anti human immunodeficiency virus agent, adult; algorithm; amino acid sequence; antiretroviral therapy; antiviral resistance; Article; comparative study; controlled study; female; gene mutation; genotype; Greece; health care delivery; human; Human immunodeficiency virus 1 infection; infection risk; major clinical study; male; McNemar test; priority journal; rural area; virus load; algorithm; antiviral resistance; genetics; Human immunodeficiency virus 1, Algorithms; Anti-HIV Agents; Drug Resistance, Viral; Greece; HIV-1; Humans
DOI:
10.1016/j.jcv.2021.104779
