Unit:
Κατεύθυνση Αναλυτική ΧημείαLibrary of the School of Science
Dissertation committee:
Αναστάσιος Οικονόμου Αναπλ. Καθηγητής ΕΚΠΑ (επιβλέπων), Μιχαήλ Κουππάρης Καθηγητής ΕΚΠΑ, Μαρία Λιούνη Αναπλ. Καθηγήτρια ΕΚΠΑ
Original Title:
Ανάπτυξη και Εφαρμογές Μεθοδολογιών Προσδιορισμού Συστατικών Πρωτογενούς Αρώματος σε Λευκούς Οίνους με Αεριοχρωματογραφία-Φασματομετρία Μαζών
Translated title:
Development and Applications of Methodologies for the Determination of Primary Aroma Compounds in White Wines with Gas Chromatography - Mass Spectrometry
Summary:
In this thesis, a SPE/GC–MS methodology and a SPME/GC–MS methodology were
developed for the determination of 21 free and glycosidically-bound volatile
varietal aroma compounds (terpenes, C6 alcohols, C13 norisoprenoids and benzene
derivatives) in wines.
Initially, a critical comparison was made of seven SPE sorbents for the
fractionation and isolation of the 21 free and bound target compounds. The most
satisfactory extraction efficiency for both free and bound fractions was
obtained with the Isolute ENV+ resin.
For the isolation/preconcentration of the target compounds with the SPME-GC/MS
method, a comparison was made of five commercially available SPME fibers in the
headspace (HS) and direct immersion (DI) modes. The fibre coated with PDMS/DVB
in the direct immersion mode was selected, as it yielded satisfactory
extraction efficiency and the best reproducibility. The statistical
significance of seven microextraction variables was evaluated using a 2-level
Plackett–Burman experimental design. Then the five statistically most
significant factors were optimised using a modified Simplex procedure.
The two methods were fully validated (in terms of selectivity, linearity,
limits of detection and quantification, recovery, repeatability,
reproducibility and uncertainty for the quantitative determination of the 21
free aroma compounds and were successfully applied to the analysis of 20
monovarietal white wine samples from indigenous and international varieties of
Vitis vinifera cultivated in Greece.
Keywords:
Primary volatile aroma compounds, White wines, Chemometry, SPE-GC/MS, SPME-GC/MS
Number of index pages:
14-19
Number of references:
284
