Unit:
ΠΜΣ με ειδίκευση ΑΠΟΜΟΝΩΣΗ, ΑΝΑΠΤΥΞΗ, ΠΑΡΑΓΩΓΗ ΚΑΙ ΕΛΕΓΧΟΣ ΒΙΟΔΡΑΣΤΙΚΩΝ ΦΥΣΙΚΩΝ ΠΡΟΪΟΝΤΩΝLibrary of the School of Science
Supervisors info:
Προκόπιος Μαγιάτης Αναπλ. Καθηγητής (Επιβλέπων),Βασίλειος Ρούσσης Καθηγητής, Ιωάννα Χήνου, Καθηγήτρια,
Original Title:
Βιοδραστικά συστατικά από τις επιτραπέζιες ελιές και τα υδατικά απόβλητα εκπίκρανσης της Μεγαριτικής ποικιλίας - ποσοτική ανάλυση με 1D και 2D NMR
Translated title:
Bioctive metabolites from "Megaritiki" table olives and debittering wastewater-quqntitative determination with 1D and 2D NMR
Summary:
Megaritiki cultivar is one of the widely used varieties as table olives.
Previous observations like the low polyphenol concentration in the
corresponding olive oil and low oleuropein concentration in olive fruits led us
to study the constituents of this variety and to investigate possible metabolic
differentiation. We aimed to study the composition of megaritiki olive fruits
and wastewaters resulting from the debittering procedure. In addition we
attempted to evaluate the recovery of bioactive metabolites from wastewater in
order to exploit this byproduct. Olives were harvested and treated with water
according to a traditional debittering method and finally placed in brine.
Wastewaters and water extract of edible olives were preconcentrated through a
selective adsorption procedure with XAD-4 resin and submitted to
chromatographic separations. Bioactive metabolites from wastewaters were
quantified using two dimensional NMR spectroscopy. The structure elucidation
was performed using NMR at 800 MHz. Wastewater contains significant amounts of
hydroxytyrosol 4-O-glucoside (35.5 mg/L), 11-methyl-oleoside (10.8 mg/L),
hydroxytyrosol (12.9 mg/L) and tyrosol (3.7 mg/L). Furthermore, wastewater
afforded two metabolites: rengyoxide and rengyoside B which are for the first
time isolated from this species. Edible olives, besides hydroxytyrosol and
tyrosol, afforded rengyoxide, haleridone and cleroindicin C which are fist
isolated from the species and four metabolites which are new natural products,
megaritodilactone, megaritolactonic acid, Megaritolactonic B methyl ester and
megaritolactonol.
Keywords:
Olive, Megaritiki, Megaritolactones, XAD-4, NMR
Number of references:
136
