Supervisors info:
Σαμανίδου Βικτωρία, Καθηγήτρια, Τμήμα Χημείας, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης
Summary:
In the present thesis, a selective method was developed and validated for the determination of six sulfonamides in milk samples. The sample's pretreatment was achieved with the use of molecularly imprinted polymer solid phase extraction, MIPSPE. The Molecularly Imprinted Polymer (MIP) that was used, has the advantage of being capable of identifying selectively six molecules-target and it can also be used for more than fifteen repetitions.
Several parameters as the conditioning of the material, the time of sample loading, the type of the elution solvent, the elution time and the steps of milk's treatment were investigated and optimized for the effective extraction of MIPSPE technique.
The developed method was validated according to the European Union Decision 657/2002/EE. The decision limits, CCα, for these six compounds are in the range between 101.9 and 106.7 μg/kg, while the results for the detection capability, CCβ, are in the range between 114.4 and 135.4 μg/kg. In addition, the quantification limits (LOQ) for these six compounds are estimated between 5.6 and 42.2 μg/kg, while the detection limits (LOD) between 1.9 and 14.1 μg/kg. As it is obvious, this method is capable of the quantitative determination and the identification of sulfonamides in milk samples which exist in lower concentrations than the maximum residue limit of 100 μg/kg which is adopted from the European Union. The suggested method is highly selective, simple in usage, sensitive and gives repeatable results. Therefore, this method can be characterized as suitable for the desired purpose.
