Unit:
Κατεύθυνση Κλινική Βιοχημεία - Μοριακή ΔιαγνωστικήLibrary of the School of Science
Author:
Papadopoulou Alexandra
Supervisors info:
Αθηνά Μάρκου, Επίκουρη Καθηγήτρια, Τμήμα Χημείας, Ε.Κ.Π.Α.
Εύη Λιανίδου, Καθηγήτρια, Τμήμα Χημείας, Ε.Κ.Π.Α.
Μαργαρίτης Αυγέρης, Αναπληρωτής Καθηγητής, Τμήμα Ιατρικής, Ε.Κ.Π.Α.
Original Title:
Μελέτη της επίδρασης του γενωμικού DNA στην ανίχνευση του HOTTIP σε δείγματα ασθενών με μεταστατικό καρκίνο του προστάτη
Translated title:
The study of the effect of genomic DNA on the detection of HOTTIP in samples from patients with metastatic prostate cancer
Summary:
In recent years, the method of liquid biopsy has attracted particular interest, as an
alternative, non-invasive approach. Liquid biobsy provides the ability to detect genetic and
epigenetic changes during disease progression, as well as to monitor patients, through
simple repeated blood sampling in real time. The main sourses of liquid biopsy are
circulating tumor cells, extracellular DNA, extracellular RNA and extracellular vesicles.
The analysis of the extracellular RNA has mainly focused on the study of circulating
miRNAs and long non-coding RNAs (lncRNAs). LncRNAs have been shown to play an
important role in gene regulation at the epigenetic, transcriptional and post-transcriptional
levels. They are also involved in the pathogenesis of tumors by acting either as oncogenes
or as tumor suppressor genes. HOTTIP is a very well-studied lncRNA that has been found
to act as an oncogene in several types of cancer including prostate cancer.
In the present study, the effect of genomic DNA on the expression of HOTTIP was
initially studied. A sensitive and specific real-time PCR method was developed to detect
HOTTIP, and the expression of HOTTIP was subsequently determined in 41 pairs of
samples - exosomes and circulating cancer cells - from patients with metastatic prostate
cancer. By using the primers listed in the majority of the published papers, it was found
that the detection rates of HOTTIP were highly elevated as the effect of genomic DNA
was not taken into account. With the developed method, HOTTIP was detected in 9.75%
of the examined CTCs and 2.43% of the corresponding exosomes.
Main subject category:
Science
Keywords:
Liquid biopsy, Metastatic prostate cancer, lncRNA, HOTTIP, genomic DNA, CTCs
Number of references:
101
