Unit:
Κατεύθυνση Φαρμακευτική Ανάλυση-Έλεγχος ΠοιότηταςLibrary of the School of Science
Author:
Stamou Panagiotis
Supervisors info:
ΠΑΝΤΕΡΗ ΕΙΡΗΝΗ, ΚΑΘΗΓΗΤΡΙΑ, Τμήμα Φαρμακευτικής, ΕΚΠΑ
ΛΟΥΚΑΣ ΙΩΑΝΝΗΣ, ΚΑΘΗΓΗΤΗΣ, Τμήμα Φαρμακευτικής, ΕΚΠΑ
ΚΟΥΠΠΑΡΗΣ ΜΙΧΑΗΛ, ΟΜ. ΚΑΘΗΓΗΤΗΣ, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Original Title:
Ανάπτυξη και επικύρωση μεθόδου εκχύλισης προσρόφησης σε υφασμάτινο μέσο με χρήση πειραματικού σχεδιασμού για τον ποσοτικό προσδιορισμό pioglitazone, repaglinide και nateglinide σε ανθρώπινο πλάσμα με την τεχνική HILIC-ESI/MS
Translated title:
Development and validation of a fabric phase sorptive extraction method via experimental design for the quantitative determination of pioglitazone, repaglinide and nateglinide in human plasma by HILIC-ESI/MS
Summary:
Type 2 diabetes mellitus is a chronic metabolic disorder characterized by the progressive dysregulation of the homeostasis mechanisms of blood glucose, resulting in the manifestation of hyperglycemia. Early symptoms include polyuria and polydipsia, while gradually the long-term complications of the disease occur due to atherosclerosis phenomena of large and small diameter vessels, leading to insufficient irrigation and dysfunction of peripheral tissues and major organs. Management of diabetes mellitus requires the application of a holistic approach, which includes appropriate lifestyle interventions (reduction of body weight, increased physical activity, etc.), in combination with the occasional administration of medication. Pioglitazone repaglinide and nateglinide constitute some indicative representatives in the group of orally administered hypoglycemic agents and are routinely prescribed in daily clinical practice to treat hyperglycemia.
Thus, in the context of the present thesis, the development of a bioanalytical method for the quantitative determination of the hypoglycemic agents pioglitazone, repaglinide and nateglinide in human plasma samples is described. Recovery of target analytes from the biological matrix was achieved through the implementation of novel microextraction technique FPSE, which utilizes permeable fabric substrates made of cellulose or other natural or synthetic textiles, that have undergone chemical coating via sol-gel technology with a hybrid adsorptive polymeric material. A Design of Experiments approach was implemented during the development of the sample
preparation method in order to assess the significance of various parameters on extraction and desorption efficiency and to optimize the recovery of the analytes. Chromatographic analysis of the compounds was performed by hydrophilic interaction liquid chromatography (HILIC) in combination with mass spectrometry. The mass detector of the chromatographic system was equipped with a single quadrupole mass analyzer and an electrospray interface. Total run time did not exceed 10 min and the linearity range of the method is 25-2000 ng mL-1 for pioglitazone, 6.25-500 ng mL-1 for repaglinide and 125-10000 ng mL-1 for nateglinide. The proposed method was evaluated in terms of its main quality characteristics and may be used to support a wide range of clinical studies regarding the monitoring of therapeutic levels of the above drugs.
Main subject category:
Science
Keywords:
Diabetes mellitus, HILIC, FPSE, Design of Experiment
Number of references:
109
