Investigation of the ability of greek fungal species to biotranform cannabinoids

Postgraduate Thesis uoadl:3365138 26 Read counter

Unit:
Specialty Biotechnology
Library of the School of Science
Deposit date:
2023-11-21
Year:
2023
Author:
Georgoula Eleni
Supervisors info:
Ζαχαρούλα Γκόνου – Ζάγκου, Επίκουρη Καθηγήτρια, Τμήμα Βιολογίας ΕΚΠΑ, (Επιβλέπουσα)
Δημήτριος Χατζηνικολάου, Αναπληρωτής Καθηγητής Τμήμα Βιολογίας ΕΚΠΑ,
Παναγούλα Κόλλια, Καθηγήτρια, Τμήμα Βιολογίας ΕΚΠΑ
Original Title:
Διερεύνηση της ικανότητας στελεχών μυκήτων της ελληνικής βιοποικιλότητας να βιομετατρέπουν κανναβινοειδή
Languages:
Greek
Translated title:
Investigation of the ability of greek fungal species to biotranform cannabinoids
Summary:
Endophytic fungi, found in Cannabis sativa, seem to be responsible for the
biotransformation of cannabinoids and the enrichment of the plant with new compounds.
However, such research data are very few and more studies are needed in order to investigate
the role of the endophytic fungi, as well as of the newly produced compounds, and the
possibility for the exploitation of the latter for the benefit of humans.
For this purpose, strains of endophytic fungi, isolated from cannabis plants of the dioecious
variety Carmagnola, were grown on PDA solid medium for one week and then sections of
mycelium were incubated in liquid nutrient medium. The cultures were inoculated with CBD
and incubated for seven days. Those that presented interesting chemical profiles were
subjected to larger scale cultures. The liquid cultures were filtered and then, they were
extracted with ethyl acetate. This procedure was repeated twice. The extracts were diluted
and analyzed by high performance liquid chromatography. This procedure was followed by
liquid gravity chromatography on a silica gel column yielding fractions that merged upon
observation of TLCs. For cleaning organic compounds of interest Semi-Preparative liquid
chromatography was performed. Fractions were checked spectroscopically with NMR.
The endophytic fungus that presented the most interesting chemical profile was
Dichotomopilus erectus. Nine metabolites were isolated from the products produced by this
fungus. These metabolites were collected from the gravity of column chromatography and
after chromatographic separations. Their identification was performed by spectroscopic data
and their pseudomolecular ions were identified by mass spectrometry. Three of the isolated
metabolites were new natural products, as molecules with this specific chemical structure are
not reported in the literature. Specifically, they were identified as 4''-hydroxydehydrocannabielsoin, 4''-hydroxy-cannabidiolaldehyde and 4′′,7-dihydroxycannabielsoin.
This fact leads to the conclusion that the endophytic fungi of cannabis have a great potential
to convert compounds and yield in large quantities, so that they can be exploited in a number
of pharmaceutical applications.
Main subject category:
Science
Keywords:
Fungi, Biotransformation, Cannabinoids
Index:
Yes
Number of index pages:
8
Contains images:
Yes
Number of references:
98
Number of pages:
121
File:
File access is restricted until 2024-11-22.

ΜΕΤΑΠΤΥΧΙΑΚΗ ΔΙΠΛΩΜΑΤΙΚΗ ΕΡΓΑΣΙΑ ΕΛΕΝΗ ΓΕΩΡΓΟΥΛΑ 11-11-23.pdf
5 MB
File access is restricted until 2024-11-22.