Τίτλος:
Extracts from the Mediterranean Food Plants Carthamus lanatus, Cichorium intybus, and Cichorium spinosum Enhanced GSH Levels and Increased Nrf2 Expression in Human Endothelial Cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The Mediterranean diet is considered to prevent several diseases. In the present study, the antioxidant properties of six extracts from Mediterranean plant foods were assessed. The extracts’ chemical composition analysis showed that the total polyphenolic content ranged from 56 to 408 GAE mg/g dw of extract. The major polyphenols identified in the extracts were quercetin,luteolin, caftaric acid, caffeoylquinic acid isomers, and cichoric acid. The extracts showed in vitro high scavenging potencyagainst ABTS•+and O2•−radicals and reducing power activity. Also, the extracts inhibited peroxyl radical-induced cleavage ofDNA plasmids. The three most potent extracts, Cichorium intybus, Carthamus lanatus, and Cichorium spinosum, inhibited OH•-induced mutations in Salmonella typhimurium TA102 cells. Moreover, C. intybus ,C. lanatus, and C. spinosum extracts increased the antioxidant molecule glutathione (GSH) by 33.4, 21.5, and 10.5% at 50μg/ml, respectively, in human endothelialEA.hy926 cells.C. intybusextract was also shown to induce in endothelial cells the transcriptional expression of Nrf2 (the majortranscription factor of antioxidant genes), as well as of antioxidant genes GCLC, GSR, NQO1, and HMOX1. In conclusion, theresults suggested that extracts from edible plants may prevent diseases associated especially with endothelium damage
Συγγραφείς:
Dimitrios Stagos,1 Dimitrios Balabanos,1 Salomi Savva,1 Zoi Skaperda,1 Alexandros Priftis,1 Efthalia Kerasioti,1 Eleni V. Mikropoulou,2 Konstantina Vougogiannopoulou,2 Sofia Mitakou,2 Maria Halabalaki,2 and Demetrios Kouretas
Περιοδικό:
Oxidative Medicine and Cellular Longevity
Εκδότης:
Hindawi Publishing Corporation
Αριθμός / τεύχος:
not applicable
Λέξεις-κλειδιά:
Mediterranean diet, polyphenols, Cichorium sp., Carthamus lanatus, antioxidant, scavenging potency, Nrf2
Κύρια θεματική κατηγορία:
Επιστήμες Υγείας
Στοιχεία έργου:
The work was funded by the“Toxicology” MSc program in the Department of Biochemistry and Biotechnology at the University of Thessaly. The current work was also sup-ported by the EU and project MediHealth-H2020-MSCA-RISE-2015—“Novel natural products for healthy ageing from Mediterranean diet and food plants of other global sources”(Proposal number: 691158) under the Horizon2020 framework
