Περίληψη:
The degree of preservation and the extractability of organic molecules in both archaeological and paleontological skeletal material has been the objective of many studies during the last decades and have shown extremely promising and interesting results (Schweitzer et al., 1997, 2007; Poulakakis et al., 2002; Schmidt-Schultz & Schultz, 2004; Dotsika et al., 2011). Studies on such molecules via various protocols of extraction as well as isotopic and spectroscopic analyses (Stathopoulou et al., 2008; Dotsika et al., 2011; Kontopoulos et al., 2019) have led to important information concerning evolutionary, environmental and diagenetic issues. Collagen is one of the most abundant proteins in skeletal material and its preservation in fossil material may indicate the preservation of other extremely important biomolecules such as DNA (Turner-Walker et al., 2008). This paper aims to present results concerning the evaluation of organic preservation as well as the extraction and quantification of collagen in samples from the Quaternary locations of Tilos and Dispilio (Greece) and Aghia Napa (Cyprus). Infrared Spectroscopy (ATR and NIR) was applied to all samples, prior to extraction in means of collagen prescreening and following extraction (where successful), to study the exact composition of the extracts and examine the correlation between collagen preservation and the samples’ diagenetic profile (Stathopoulou et al., 2008; Kuczumow et al., 2010; Cleland et al., 2015; Stathopoulou et al., 2019). The extraction of collagen was attempted via modifications of the Longin (1971) method and specifically those of Maspero et al. (2011), Semal and Orban (1995) and Ambrose (1990). According to our results, only the Ambrose method led to successful collagen extraction and specifically only in the Dispilio samples. The collagen yield values for this material varied significantly (0,3–6,1% w/w) and seemed to strongly correlate to the different contexts found within the archaeological site. The IR analysis of the extracted Dispilio collagen indicated the presence of impurities such as carbonates and subsequently raised questions relating to the efficacy of the extraction method. Issues concerning geochemical and diagenetic parameters within the strata of the waterlogged site of Dispilio that could be connected to the different collagen yield as well as the methodological problems that emerged during the attempted extraction in all samples are discussed as well as the applicability of these methods on samples with reduced organic content. © 2022 Elsevier Ltd and INQUA
