Τίτλος:
Charting the structural and thermodynamic determinants in phenolic acid natural product - cyclodextrin encapsulations
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Cyclodextrins are pliable platforms that have served to optimize the pharmaceutic profile of numerous compounds and to enhance the stability of natural food additives. Caffeic and rosmarinic acid are natural products with proven health benefits, though their full therapeutic potential has not been exploited. To enhance their pharmaceutic profile, we developed cyclodextrin-based formulates and unveiled their thermodynamic and structural principles. The complexes' stoichiometry was determined by ESI-MS. Solid-state and liquid NMR spectroscopy revealed the interactions and the topographical location of the caffeic and rosmarinic acid inside the cyclodextrin cavity. The theoretically analyzed HP-β-CD's degree of substitution (DS) of caffeic and rosmarinic acids can explain the intensities obtained by 2D NOESY experiments. The thermodynamics and the affinity of the complexes were evaluated through isothermal titration calorimetry. In addition, the rosmarinic and caffeic acids as, also, their complexes showed considerable antimicrobial activity against common food spoilage and pathogenic bacteria. The generated data could provide the basis to understand the structural and thermodynamic determinants implicated in natural products - CD recognition and to develop platforms for the optimization of their pharmaceutical and stability profiles in order to be utilized as safe and stable natural antimicrobial food additives.Communicated by Ramaswamy H. Sarma.
Συγγραφείς:
I. Andreadelis, M.V. Chatziathanasiadou, D. Ntountaniotis, A.G. Tzakos, T. Mavromoustakos
Περιοδικό:
Journal of Biomolecular Structure and Dynamics
Εκδότης:
TAYLOR AND FRANCIS
Λέξεις-κλειδιά:
2-hydroxypropyl-β-cyclodextrin; Caffeic acid; NMR spectroscopy; antimicrobial activity; isothermal titration calorimetry; molecular dynamics; rosmarinic acid
Κύρια θεματική κατηγορία:
Θετικές Επιστήμες
DOI:
10.1080/07391102.2020.1751716
Αρχείο:
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10.1080@07391102.2020.1751716 (1).pdf
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