Τίτλος:
Functional analysis of 3'UTR polymorphisms in the caprine SLC11A1 gene and its association with the Mycobacterium avium subsp. paratuberculosis infection
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The study aimed to investigate whether the genetic polymorphisms in the 3. 'UTR of the caprine SLC11A1 gene are functional, and to assess the role of MAP as a regulatory parameter in gene expression. To this goal we constructed plasmids expressing the Luciferase reporter gene in transient transfections of a mouse (Balb/c) macrophage cell line (RAW264.7), incorporating those polymorphisms that our previous work indicated as more prominent in terms of SLC11A1 expression and responsiveness to MAP infection. Gene expression variation was recorded on the average of the respective measurements after exposure to Mycobacterium avium subsp. paratuberculosis (MAP) combined with microbial antigens and cytokines. In silico analysis of the region under study allowed identification of one cis-acting RNA element, five putative transcriptional regulatory elements and 85 3. 'end microRNA binding sites. The two polymorphic regions (regions A and B) of the 3. 'UTR of the caprine SLC11A1 gene were recognized as regulators of its activity, at transcriptional and post-transcriptional level. The GT16 polymorphism at region A, combined with the GT8 polymorphism at region B, results in up-regulation of the SLC11A1 gene. The specific genotype was also found to be more responsive to MAP exposure at a statistically significant level. © 2015 Elsevier B.V.
Συγγραφείς:
Taka, S.
Gazouli, M.
Sotirakoglou, K.
Liandris, E.
Andreadou, M.
Triantaphyllopoulos, K.
Ikonomopoulos, J.
Περιοδικό:
Veterinary Immunology and Immunopathology
Λέξεις-κλειδιά:
bacterial antigen; cis acting element; cytokine; gamma interferon; luciferase; microRNA; natural resistance associated macrophage protein 1; 3' untranslated region; cation transport protein; natural resistance-associated macrophage protein 1, 3' untranslated region; animal cell; animal experiment; animal model; Article; binding site; computer model; controlled study; expression vector; functional assessment; gene activity; gene expression; gene expression regulation; gene function; genetic association; genetic polymorphism; genetic transcription; genetic variability; genotype; goat; luciferase assay; macrophage; mouse; Mycobacterium avium subsp. paratuberculosis; nonhuman; paratuberculosis; plasmid; regulatory sequence; reporter gene; RNA binding; RNA processing; transient transfection; upregulation; animal; genetics; goat disease; host pathogen interaction; immunology; microbiology; paratuberculosis; pathogenicity; RAW 264.7 cell line, Capra; Mycobacterium avium subsp. paratuberculosis, 3' Untranslated Regions; Animals; Cation Transport Proteins; Gene Expression Regulation; Goat Diseases; Goats; Host-Pathogen Interactions; Macrophages; Mice; Mycobacterium avium subsp. paratuberculosis; Paratuberculosis; Polymorphism, Genetic; RAW 264.7 Cells
DOI:
10.1016/j.vetimm.2015.06.004
