Τίτλος:
Identification of two novel circular rnas deriving from bcl2l12 and investigation of their potential value as a molecular signature in colorectal cancer
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The utility of circular RNAs (circRNAs) as molecular biomarkers has recently emerged. However, only a handful of them have already been studied in colorectal cancer (CRC). The purpose of this study was to identify new circRNAs deriving from BCL2L12, a member of the BCL2 apoptosis-related family, and investigate their potential as biomarkers in CRC. Total RNA extracts from CRC cell lines and tissue samples were reversely transcribed. By combining PCR with divergent primers and nested PCR followed by Sanger sequencing, we were able to discover two BCL2L12 circRNAs. Subsequently, bioinformatical tools were used to predict the interactions of these circRNAs with microRNAs (miRNAs) and RNA-binding proteins (RBPs). Following a PCR-based pre-amplification, real-time qPCR was carried out for the quantification of each circRNA in CRC samples and cell lines. Biostatistical analysis was used to assess their potential prognostic value in CRC. Both novel BCL2L12 circRNAs likely interact with particular miRNAs and RBPs. Interestingly, circ-BCL2L12-2 expression is inversely associated with TNM stage, while circ-BCL2L12-1 overexpression is associated with shorter overall survival in CRC, particularly among TNM stage II patients. Overall, we identified two novel BCL2L12 circRNAs, one of which can further stratify TNM stage II patients into two subgroups with substantially distinct prognosis. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Συγγραφείς:
Karousi, P.
Artemaki, P.I.
Sotiropoulou, C.D.
Christodoulou, S.
Scorilas, A.
Kontos, C.K.
Περιοδικό:
International Journal of Molecular Sciences
Λέξεις-κλειδιά:
circular ribonucleic acid; microRNA; protein bcl 2; protein bcl x; RNA binding protein; BCL2L12 protein, human; microRNA; muscle protein; protein bcl 2; RNA binding protein; tumor marker, alternative RNA splicing; apoptosis; Article; bcl2l12 gene; cancer staging; colorectal cancer; gene; gene overexpression; human; human cell; human tissue; in vitro study; nested polymerase chain reaction; overall survival; protein expression; real time polymerase chain reaction; Sanger sequencing; transcriptomics; adult; aged; biology; blood; colorectal tumor; female; gene expression regulation; genetics; male; middle aged; pathology; very elderly, Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; Colorectal Neoplasms; Computational Biology; Female; Gene Expression Regulation, Neoplastic; Humans; Male; MicroRNAs; Middle Aged; Muscle Proteins; Proto-Oncogene Proteins c-bcl-2; RNA, Circular; RNA-Binding Proteins
DOI:
10.3390/ijms21228867
