Τίτλος:
Chitosan/gelatin scaffolds support bone regeneration
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Chitosan/Gelatin (CS:Gel) scaffolds were fabricated by chemical crosslinking with glutaraldehyde or genipin by freeze drying. Both crosslinked CS:Gel scaffold types with a mass ratio of 40:60% form a gel-like structure with interconnected pores. Dynamic rheological measurements provided similar values for the storage modulus and the loss modulus of the CS:Gel scaffolds when crosslinked with the same concentration of glutaraldehyde vs. genipin. Compared to genipin, the glutaraldehyde-crosslinked scaffolds supported strong adhesion and infiltration of pre-osteoblasts within the pores as well as survival and proliferation of both MC3T3-E1 pre-osteoblastic cells after 7 days in culture, and human bone marrow mesenchymal stem cells (BM-MSCs) after 14 days in culture. The levels of collagen secreted into the extracellular matrix by the pre-osteoblasts cultured for 4 and 7 days on the CS:Gel scaffolds, significantly increased when compared to the tissue culture polystyrene (TCPS) control surface. Human BM-MSCs attached and infiltrated within the pores of the CS:Gel scaffolds allowing for a significant increase of the osteogenic gene expression of RUNX2, ALP, and OSC. Histological data following implantation of a CS:Gel scaffold into a mouse femur demonstrated that the scaffolds support the formation of extracellular matrix, while fibroblasts surrounding the porous scaffold produce collagen with minimal inflammatory reaction. These results show the potential of CS:Gel scaffolds to support new tissue formation and thus provide a promising strategy for bone tissue engineering. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
Συγγραφείς:
Georgopoulou, A.
Papadogiannis, F.
Batsali, A.
Marakis, J.
Alpantaki, K.
Eliopoulos, A.G.
Pontikoglou, C.
Chatzinikolaidou, M.
Περιοδικό:
Journal of Materials Science: Materials in Medicine
Εκδότης:
Springer New York LLC
Λέξεις-κλειδιά:
Bone; Cell culture; Chitosan; Collagen; Crosslinking; Digital storage; Flowcharting; Gene expression; Osteoblasts; Stem cells; Tissue; Tissue culture, Bone tissue engineering; Chemical cross-linking; Dynamic rheological measurements; Extracellular matrices; Human bone marrow mesenchymal stem cells; Inflammatory reaction; Interconnected pores; Tissue-culture polystyrenes, Scaffolds (biology), 5' nucleotidase; chitosan; collagen; endoglin; gelatin; genipin; glutaraldehyde; polystyrene; Thy 1 membrane glycoprotein; transcription factor RUNX2; biomaterial; chitosan; gelatin, adipocyte; animal experiment; animal tissue; Article; bone marrow derived mesenchymal stem cell; bone regeneration; cell adhesion; cell culture; cell differentiation; cell infiltration; cell interaction; cell isolation; cell proliferation; cell surface; cell survival; cell viability; controlled study; degradation; extracellular matrix; flow kinetics; gene expression; histology; human; human cell; immunophenotyping; in vitro study; monolayer culture; mouse; nonhuman; ossification; osteoblast; osteocyte; priority journal; reverse transcription polymerase chain reaction; RNA extraction; scanning electron microscopy; spectroscopy; tissue engineering; water transport; animal; bone development; bone marrow cell; chemistry; cytology; devices; drug effect; materials testing; mesenchymal stroma cell; physiology; procedures; synthesis; tissue scaffold, Animals; Biocompatible Materials; Bone Marrow Cells; Bone Regeneration; Cell Differentiation; Cell Proliferation; Cells, Cultured; Chitosan; Gelatin; Humans; Materials Testing; Mesenchymal Stromal Cells; Mice; Osteoblasts; Osteogenesis; Tissue Engineering; Tissue Scaffolds
DOI:
10.1007/s10856-018-6064-2
