Τίτλος:
New SPE loading material for affinity-based separation of cyclodextrins from drug:CD complexes in order to overcome Beer's law deviations
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Molecular inclusion of guest molecules within CDs is known to alter guest molecule spectrophotometric absorptivity, making their determination, based on spectrophotometric data, inaccurate. Therefore specific analytical methods capable of quantifying the drugs as free molecules must be developed and validated. SPE was selected to simplify sample and avoid more time-consuming alternatives. A new solid phase was synthesized and characterized by infrared spectrometry, differential scanning calorimetry and elemental analysis. The competitive complexation of adamantane groups immobilized on the silica substrate facilitates drug:CD complex dissociation and elimination of CD from samples. The drug molecules, now free from CD, can be easily analysed by an already available HPLC method. This new SPE loading material was employed in the determination of ketoprofen in its CD complex as a representative example of the utility of this novel material. The calculated analytical errors were reduced from a maximum of 20.79% (without SPE) to a minimum of 3.99%. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
Συγγραφείς:
Rozou, S.
Raftopoulos, P.
Hatziantoniou, S.
Antoniadou-Vyza, E.
Περιοδικό:
Journal of High Resolution Chromatography (now called Journal of Separation Science)
Λέξεις-κλειδιά:
Absorptivities; Adamantanes; Affinity-based separation; Analytical errors; Analytical method; Beer's law; Cd complex; CdS; Drug molecules; Elemental analysis; Free molecules; Guest molecules; HPLC method; Infrared spectrometry; Ketoprofen; Novel materials; Sample pretreatment; Silica substrate; Solid-phase; SPE; Stationary phase, Beverages; Complexation; Cyclodextrins; Differential scanning calorimetry; Fluorine containing polymers; Infrared spectroscopy; Molecules; Silanes; Silica; Solar radiation; Spectrophotometers; Spectrophotometry, Data storage equipment, cyclodextrin; ketoprofen; silicon dioxide, analytical error; article; calculation; calorimetry; drug formulation; drug structure; drug synthesis; high performance liquid chromatography; infrared spectroscopy; material state; phase separation; priority journal; separation technique; solid phase extraction; temperature, Calorimetry, Differential Scanning; Cyclodextrins; Drug Carriers; Molecular Structure; Pharmaceutical Preparations; Polymers; Pyrans; Solid Phase Extraction; Spectroscopy, Fourier Transform Infrared
DOI:
10.1002/jssc.200900251
