Τίτλος:
Assessment of the nutraceutical effects of oleuropein and the cytotoxic effects of adriamycin, when administered alone and in combination, in mg-63 human osteosarcoma cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Oleuropein (OLEU) is the most distinguished phenolic compound found in olive fruit and the leaves of Olea europaea L., with several pharmacological properties, including anti-cancer actions. Adriamycin (ADR) is an anthracycline widely used as a chemotherapeutic agent, although it presents significant side effects. The aim of the present study was to investigate the effect of oleuropein alone (20 µg/mL) and in co-treatment with ADR (50 nM), in MG-63 human osteosarcoma cells. Therefore, cellular and molecular techniques, such as MTT assay, flow cytometry, real- time Polymerase Chain Reaction (PCR), western blot and Elisa method, as well as Nuclear Magnetic Resonance (NMR) spectroscopy, were applied to unveil changes in the signal transduction pathways involved in osteosarcoma cells survival. The observed alterations in gene, protein and metabolite levels denote that OLEU not only inhibits MG-63 cells proliferation and potentiates ADR’s cytotoxicity, but also exerts its action, at least in part, through the induction of autophagy. © 2021 by the authors.
Συγγραφείς:
Gioti, K.
Papachristodoulou, A.
Benaki, D.
Aligiannis, N.
Skaltsounis, A.-L.
Mikros, E.
Tenta, R.
Περιοδικό:
Nutrient Cycling in Agroecosystems
Λέξεις-κλειδιά:
doxorubicin; messenger RNA; nutraceutical; oleuropein; serine threonine protein kinase ULK1; antiinfective agent; antineoplastic antibiotic; cytotoxin; doxorubicin; iridoid; oleuropein, analytic method; antiproliferative activity; Article; autophagy (cellular); cell cycle phase; cell survival; controlled study; DNA content; drug cytotoxicity; drug mechanism; drug potentiation; enzyme linked immunosorbent assay; flow cytometry; gene expression level; human; human cell; IC50; mass spectrometry; metabolic fingerprinting; metabolite; MG-63 cell line; mRNA expression level; MTT assay; nuclear magnetic resonance spectroscopy; osteosarcoma cell; real time polymerase chain reaction; signal transduction; upregulation; Western blotting; cell proliferation; dietary supplement; drug effect; osteosarcoma; tumor cell culture, Anti-Infective Agents; Antibiotics, Antineoplastic; Cell Proliferation; Cell Survival; Cytotoxins; Dietary Supplements; Doxorubicin; Humans; Iridoids; Osteosarcoma; Tumor Cells, Cultured
