Περίληψη:
Objectives: The purpose of the study was to evaluate the integrity of dentine type I collagen after self-etching (SE) treatments with strong and mild universal adhesives. Methods: Coronal dentine specimens (n = 10/product) were imaged by optical microscopy and analyzed by ATR-FTIR spectroscopy before and after treatment with 32% phosphoric acid gel (PA-negative control), 17% neutral EDTA (ED-positive control) conditioners and Adhese Universal (AD), Clearfil Universal Bond Quick (CQ), G-Premio Bond (GP), Prelude One (PR) and Scotchbond Universal (SB) adhesives. From the spectroscopic analysis the following parameters were determined: a) Extent of dentine demineralization (DM%) and b) percentage area of the Amide I curve-fitted components of β-turns, 310-helix/β-turns, α-helix, random coils, β-sheets and collagen maturation (R) index. Statistical analysis was performed by one-way ANOVA (DM%), paired t-test/Wilcoxon test (Amide I components) and Spearman correlation coefficient (DM% vs Amide I components) at an a = 0.05 level. Results: PA, ED and GP removed the smear-layer and opened tubule orifices, whereas all other treatments removed only the intratubular smear-layer fraction. The ranking of the statistically significant differences in DM% was PA > GP > ED > AD, SB, CQ, PR, with AD being significantly different from PR. Regarding the Amide I components, PA demonstrated a significant reduction in β-turns, α-helices and an increase in β-sheets, GP a reduction in β-turns, AD an increase in β-turns and random coils, and CQ an increase in β-turns. PR, SB and ED showed insignificant differences in all the Amide I components. Significant correlations were found between DM%-random coils and DM%-R. Significance: The universal adhesives used in the SE mode induced none to minimal changes in dentine collagen structure, without evidence of the destabilization pattern observed after conventional phosphoric acid treatments. © 2021 The Academy of Dental Materials
Συγγραφείς:
Anastasiadis, K.
Verdelis, K.
Eliades, G.
Λέξεις-κλειδιά:
Amides; Collagen; Etching; Fourier transform infrared spectroscopy; Phosphoric acid; Reduction; Spectroscopic analysis, After-treatment; ATR FT-IR spectroscopies; Collagen structure; Negative control; Phosphoric acid treatments; Spearman correlation coefficients; Statistically significant difference; Universal adhesives, Adhesives, collagen; dentin bonding agent; resin cement; tooth cement, dental acid etching; dental bonding; dentin; materials testing; surface property; tensile strength, Acid Etching, Dental; Collagen; Dental Bonding; Dental Cements; Dentin; Dentin-Bonding Agents; Materials Testing; Resin Cements; Surface Properties; Tensile Strength
