Τίτλος:
Regulation of matrix metalloproteinase-1 by Filifactor alocis in human gingival and monocytic cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objectives: Periodontitis is a highly prevalent chronic inflammatory disease caused by periodontopathogens, such as Filifactor alocis. This study sought to examine the matrix metalloproteinase (MMP)-1 synthesis by monocytic and fibroblastic cells in response to F. alocis and to unravel the underlying cellular mechanisms. Material and methods: Gingival biopsies from periodontally healthy and periodontitis individuals were analyzed for the presence of F. alocis and MMP-1 by RT-PCR. Human gingival fibroblastic (HGF-1) and monocytic (THP-1) cells were stimulated with F. alocis in the presence and absence of a blocking toll-like receptor (TLR)2 antibody or specific inhibitors against MAPKs. MMP-1 expression and protein levels were studied by RT-PCR and ELISA, respectively. Results: F. alocis was highly prevalent in biopsies from periodontitis patients but barely present in the healthy gingiva. Significantly higher MMP-1 expression levels were found in the inflamed gingiva as compared with healthy biopsies. F. alocis caused a significant and dose-dependent MMP-1 upregulation in both cells. The stimulatory effect of F. alocis on MMP-1 was TLR2- and MAPK-dependent and more pronounced on THP-1 cells as compared with HGF-1 cells. Conclusions: Our results demonstrate that F. alocis and MMP-1 are more prevalent at periodontitis sites. Additionally, our study provides original evidence that F. alocis can stimulate MMP-1 production by fibroblastic and monocytic cells, suggesting that F. alocis may contribute to periodontal breakdown through MMP-1. Clinical relevance: F. alocis and MMP-1 are linked to each other and key players in periodontitis, which may have significant implications for future diagnostic and treatment strategies. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
Συγγραφείς:
Nokhbehsaim, M.
Nogueira, A.V.B.
Damanaki, A.
Dalagiorgou, G.
Eick, S.
Adamopoulos, C.
Piperi, C.
Basdra, E.K.
Papavassiliou, A.G.
Deschner, J.
Περιοδικό:
Clinical Oral Investigations
Λέξεις-κλειδιά:
interstitial collagenase, Clostridiales; fibroblast; gingiva; human; metabolism; microbiology; periodontitis; physiology, Clostridiales; Fibroblasts; Gingiva; Humans; Matrix Metalloproteinase 1; Periodontitis
DOI:
10.1007/s00784-019-03062-0
