Τίτλος:
Continuous hydrostatic pressure induces differentiation phenomena in chondrocytes mediated by changes in polycystins, SOX9, and RUNX2 [Kontinuierlicher hydrostatischer Druck induziert Differenzierungsphänomene in Chondrozyten durch Änderungen in Polyzystinen, SOX9 und RUNX2]
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Purpose: The present study aimed to investigate the long-term effects of hydrostatic pressure on chondrocyte differentiation, as indicated by protein levels of transcription factors SOX9 and RUNX2, on transcriptional activity of SOX9, as determined by pSOX9 levels, and on the expression of polycystin-encoding genes Pkd1 and Pkd2. Materials and methods: ATDC5 cells were cultured in insulin-supplemented differentiation medium (ITS) and/or exposed to 14.7 kPa of hydrostatic pressure for 12, 24, 48, and 96 h. Cell extracts were assessed for SOX9, pSOX9, and RUNX2 using western immunoblotting. The Pkd1 and Pkd2 mRNA levels were detected by real-time PCR. Results: Hydrostatic pressure resulted in an early drop in SOX9 and pSOX9 protein levels at 12 h followed by an increase from 24 h onwards. A reverse pattern was followed by RUNX2, which reached peak levels at 24 h of hydrostatic pressure-treated chondrocytes in ITS culture. Pkd1 and Pkd2 mRNA levels increased at 24 h of combined hydrostatic pressure and ITS treatment, with the latter remaining elevated up to 96 h. Conclusions: Our data indicate that long periods of continuous hydrostatic pressure stimulate chondrocyte differentiation through a series of molecular events involving SOX9, RUNX2, and polycystins-1, 2, providing a theoretical background for functional orthopedic mechanotherapies. © 2016, Springer-Verlag Berlin Heidelberg.
Συγγραφείς:
Karamesinis, K.
Spyropoulou, A.
Dalagiorgou, G.
Katsianou, M.A.
Nokhbehsaim, M.
Memmert, S.
Deschner, J.
Vastardis, H.
Piperi, C.
Περιοδικό:
Journal of Orofacial Orthopedics/Fortschritte der Kieferorthopδdie
Εκδότης:
Urban und Vogel GmbH
Λέξεις-κλειδιά:
Runx2 protein, mouse; Sox9 protein, mouse; transcription factor RUNX2; transcription factor Sox9, animal; cell differentiation; cell line; chondrocyte; chondrogenesis; cytology; gene expression regulation; hydrostatic pressure; mechanical stress; mechanotransduction; metabolism; mouse; physiology, Animals; Cell Differentiation; Cell Line; Chondrocytes; Chondrogenesis; Core Binding Factor Alpha 1 Subunit; Gene Expression Regulation, Developmental; Hydrostatic Pressure; Mechanotransduction, Cellular; Mice; SOX9 Transcription Factor; Stress, Mechanical
DOI:
10.1007/s00056-016-0061-1
