Τίτλος:
Absence of hotspot mutations in exons 9 and 20 of the PIK3CA gene in human oral squamous cell carcinoma in the Greek population
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objective: Phosphatidylinositol-3 kinases (PI3K) are a group of heterodimeric lipid kinases that regulate many cellular processes. Recent studies have reported high frequencies of somatic hotspot mutations in the phosphatidylinositol-3 kinase catalytic α (PIK3CA) gene, which encodes for one of these kinases, in several human solid tumors, including oral squamous cell carcinoma (OSCC). The aim of this study was to determine the frequency of hotspot mutations in exons 9 and 20 of the PIK3CA gene in OSCC in the Greek population. Study design: Eighty-six formalin-fixed and paraffin-embedded primary tumor specimens were analyzed by direct genomic DNA sequencing. Chi-square was used for statistical analysis. Results: No hotspot mutations were detected in any of the samples. Two intronic polymorphisms IVS8 and IVS9 were detected, mainly in patients with cancer of the buccal mucosa and lower gingival and alveolus respectively. Conclusions: PIK3CA hotspot mutations are unlikely to play a major role in the pathogenesis of OSCC in the Greek population. © 2010 Mosby, Inc. All rights reserved.
Συγγραφείς:
Kostakis, G.C.
Papadogeorgakis, N.
Koumaki, V.
Kamakari, S.
Koumaki, D.
Alexandridis, C.
Περιοδικό:
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
Λέξεις-κλειδιά:
cytosine; guanine; phosphatidylinositol 3 kinase; PIK3CA protein, human; thymine; tumor marker, adult; aged; article; cancer staging; case control study; comparative study; DNA sequence; enzymology; exon; female; genetic polymorphism; genetics; gingiva tumor; Greece; human; intron; male; mandible tumor; middle aged; mouth mucosa; mouth tumor; mutation; pathology; polymerase chain reaction; squamous cell carcinoma; tongue tumor, 1-Phosphatidylinositol 3-Kinase; Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell; Case-Control Studies; Cytosine; Exons; Female; Gingival Neoplasms; Greece; Guanine; Humans; Introns; Male; Mandibular Neoplasms; Middle Aged; Mouth Mucosa; Mouth Neoplasms; Mutation; Neoplasm Staging; Polymerase Chain Reaction; Polymorphism, Genetic; Sequence Analysis, DNA; Thymine; Tongue Neoplasms; Tumor Markers, Biological; Young Adult
DOI:
10.1016/j.tripleo.2010.01.015
