Τίτλος:
Effect of prolactin in the absence of hCG on maturation, fertilization, and embryonic development of in vitro matured mouse oocytes
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Oocyte maturation is a complex process involving both the progression of meiotic cycle and the reprogramming of cytoplasmic events. The aim of this study was to investigate the effects of prolactin (PRL) in the in vitro maturation (IVM) of preantral mouse oocytes, in the absence of human chorionic gonadotrophin (hCG). Mouse preantral follicles were collected from female mice without prior hormonal ovarian stimulation and were cultured in the presence of varying concentrations of PRL (20, 100, 200, and 300 ng/mL) for 12 days. A group of in vitro matured oocytes were assessed for polar body (PB) formation, while the rest were fertilized and embryonic development was recorded. The maturation of preantral mouse follicles, as well as their fertilization and cleavage rates, observed when the culture mediumwas supplemented with middle- and high-range doses of PRL was beneficially affected. This effect was considerably high, although the culture media lacked hCG, a hormone extensively used in modern ovulation induction regiments, as well as in IVM media. © 2006 New York Academy of Sciences.
Συγγραφείς:
Kiapekou, E.
Zapanti, E.
Mastorakos, G.
Beretsos, P.
Bletsa, R.
Drakakis, P.
Loutradis, D.
Antsaklis, A.
Περιοδικό:
Annals of the New York Academy of Sciences
Εκδότης:
Blackwell Publishing Inc.
Λέξεις-κλειδιά:
chorionic gonadotropin; prolactin; prolactin, animal cell; animal experiment; cell culture; cell maturation; concentration (parameters); conference paper; controlled study; culture medium; embryo development; fertilization; hormone action; maturation; mouse; nonhuman; oocyte; ovary follicle; ovulation; animal; article; culture technique; dose response; drug effect; female; oocyte; ovary follicle; ovulation induction; physiology, Mus, Animals; Chorionic Gonadotropin; Culture Media; Culture Techniques; Dose-Response Relationship, Drug; Female; Mice; Oocytes; Ovarian Follicle; Ovulation Induction; Prolactin
DOI:
10.1196/annals.1365.044
