Περίληψη:
β-Amyloid (Aβ) related pathology shows a range of lesions which differ both qualitatively and quantitatively. Pathologists, to date, mainly focused on the assessment of both of these aspects but attempts to correlate the findings with clinical phenotypes are not convincing. It has been recently proposed in the same way as 1 and α synuclein related lesions, also Aβ related pathology may follow a temporal evolution, i.e. distinct phases, characterized by a step-wise involvement of different brain-regions. Twenty-six independent observers reached an 81% absolute agreement while assessing the phase of Aβ, i.e. phase 1 = deposition of Aβ exclusively in neocortex, phase 2 = additionally in allocortex, phase 3 = additionally in diencephalon, phase 4 = additionally in brainstem, and phase 5 = additionally in cerebellum. These high agreement rates were reached when at least six brain regions were evaluated. Likewise, a high agreement (93%) was reached while assessing the absence/presence of cerebral amyloid angiopathy (CAA) and the type of CAA (74%) while examining the six brain regions. Of note, most of observers failed to detect capillary CAA when it was only mild and focal and thus instead of type 1, type 2 CAA was diagnosed. In conclusion, a reliable assessment of Aβ phase and presence/absence of CAA was achieved by a total of 26 observers who examined a standardized set of blocks taken from only six anatomical regions, applying commercially available reagents and by assessing them as instructed. Thus, one may consider rating of Aβ-phases as a diagnostic tool while analyzing subjects with suspected Alzheimer's disease (AD). Because most of these blocks are currently routinely sampled by the majority of laboratories, assessment of the Aβ phase in AD is feasible even in large scale retrospective studies. © Springer-Verlag 2009.
Συγγραφείς:
Alafuzoff, I.
Thal, D.R.
Arzberger, T.
Bogdanovic, N.
Al-Sarraj, S.
Bodi, I.
Boluda, S.
Bugiani, O.
Duyckaerts, C.
Gelpi, E.
Gentleman, S.
Giaccone, G.
Graeber, M.
Hortobagyi, T.
Höftberger, R.
Ince, P.
Ironside, J.W.
Kavantzas, N.
King, A.
Korkolopoulou, P.
Kovács, G.G.
Meyronet, D.
Monoranu, C.
Nilsson, T.
Parchi, P.
Patsouris, E.
Pikkarainen, M.
Revesz, T.
Rozemuller, A.
Seilhean, D.
Schulz-Schaeffer, W.
Streichenberger, N.
Wharton, S.B.
Kretzschmar, H.
Λέξεις-κλειδιά:
amyloid beta protein, adult; aged; Alzheimer disease; amyloidosis; article; brain region; brain stem; cerebellum; clinical article; diencephalon; disease course; female; human; human tissue; immunohistochemistry; laboratory test; male; neocortex; olfactory cortex; priority journal; protein analysis; protein blood level; vascular amyloidosis, Aged; Aged, 80 and over; Alzheimer Disease; Amyloid beta-Protein; Brain; Cerebral Amyloid Angiopathy; Cerebral Arteries; Disease Progression; Female; Humans; Immunohistochemistry; Male; Middle Aged; Reference Values; Reproducibility of Results
