Τίτλος:
The effects of rivastigmine plus selegiline on brain acetylcholinesterase, (Na+, K+)-, Mg2+- ATPase activities, antioxidant status, and learning performance of aged rats
Περίληψη:
We investigated the effects of rivastigmine (a cholinesterase inhibitor) and selegiline ((-)deprenyl, an irreversible inhibitor of monoamineoxidase-B), alone and in combination, on brain acetylcholinesterase (AChE), (Na+, K+)-, Mg2+-ATPase activities, total antioxidant status (TAS), and learning performance, after long-term drug administration in aged male rats. The possible relationship between the biochemical and behavioral parameters was evaluated. Methods: Aged rats were treated (for 36 days) with rivastigmine (0.3 mg/ kg rat/day ip), selegiline (0.25 mg/kg rat/day im), rivastigmine plus selegiline in the same doses and way of administration as separately. Aged and adult control groups received NaCl 0.9% 0.5 ml ip. Results: TAS was lower in aged than in adult rats, rivastigmine alone does not affect TAS, decreases AChE activity, increases (Na+, K+)-ATPase and Mg2+-ATPase activity of aged rat brain and improves cognitive performance. Selegiline alone decreases free radical production and increases AChE activity and (Na+, K+)-ATPase activity, improving cognitive performance as well. In the combination: rivastigmine seems to cancel selegiline action on TAS and AChE activity, while it has additive effect on (Na+, K+)-ATPase activity. In the case of Mg2+-ATPase selegiline appears to attenuate rivastigmine activity. No statistically significant difference was observed in the cognitive performance. Conclusion: Reduced TAS, AChE activity and learning performance was observed in old rats. Both rivastigmine and selesiline alone improved performance, although they influenced the biochemical parameters in a different way. The combination of the two drugs did not affect learning performance. © 2008 Carageorgiou et al, publisher and licensee Dove Medical Press Ltd.
Συγγραφείς:
Carageorgiou, H.
Sideris, A.C.
Messari, I.
Liakou, C.I.
Tsakiris, S.
Λέξεις-κλειδιά:
acetylcholinesterase; adenosine triphosphatase (magnesium); adenosine triphosphatase (potassium sodium); free radical; norselegiline; rivastigmine; sodium chloride, age distribution; aged; animal behavior; animal experiment; animal tissue; antioxidant activity; article; biochemistry; brain function; chronic drug administration; cognition; combination chemotherapy; controlled study; drug potentiation; enzyme activation; enzyme activity; enzyme inhibition; learning; male; monotherapy; nonhuman; passive avoidance; rat; recognition; statistical significance
