Τίτλος:
Investigation of the epitopic structure of thymosin β10 by epitope mapping experiments
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
We present here a study on the epitopic structure and the immunochemical characteristics of thymosin β10 (Tβ10), a 43 aminoacid peptide involved in important cellular mechanisms, by using the epitope mapping Multipin method. Octapeptides overlapping by one amino acid so as to represent the whole sequence of Tβ10 were synthesized on polystyrene pins and screened, using an ELISA method, with a polyclonal antiserum raised against intact recombinant Tβ10. The octapeptides were also tested with anti-peptide oligoclonal antisera raised against the synthetic fragments Tβ10[1-16] and Tβ10[31-43], with polyclonal antisera raised against natural thymosin β4 (Tβ4) or thymosin β9 (Tβ9), and with anti-peptide oligoclonal antisera raised against various fragments of Tβ4 (i.e. Tβ4[1-11], Tβ4[30-43] and Tβ4[16-38]). Four distinct epitopic fragments were revealed, namely the sequences 1-13, 19-30, 29-40 and 36-43. Among them, the sequence 36-43 appears to offer unique immunochemical characteristics to the Tβ10 molecule. Copyright (C) 1999 Elsevier Science Inc.
Συγγραφείς:
Vassiliadou, I.
Leondiadis, L.
Ferderigos, N.
Ithakissios, D.S.
Evangelatos, G.P.
Livaniou, E.
Λέξεις-κλειδιά:
peptide; thymosin; thymosin beta10; unclassified drug, article; enzyme linked immunosorbent assay; epitope mapping; human; immunochemistry; priority journal, Epitope Mapping; Epitopes; Recombinant Proteins; Thymosin
DOI:
10.1016/S0196-9781(98)00169-7
