Τίτλος:
The development of various forms of lung injury with increasing tidal volume in normal rats
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Sixty-three, open-chest normal rats were subjected to mechanical ventilation (MV) with tidal volumes (VT) ranging from 7.5–39.5ml kg−1 and PEEP 2.3 cmH2O. Arterial blood gasses and pressure, and lung mechanics were measured during baseline ventilation (VT = 7.5ml kg−1) before and after test ventilation, when cytokine, von Willebrand factor (vWF), and albumin concentration in serum and broncho-alveolar lavage fluid (BALF), wet-to-dry weight ratio (W/D), and histologic injury scores were assessed. Elevation of W/D and serum vWF and cytokine concentration occurred with VT > 25ml kg−1. With VT > 30ml kg−1 cytokine and albumin concentration increased also in BALF, arterial oxygen tension decreased, lung mechanics and histology deteriorated, while W/D and vWF and cytokine concentration increased further. Hence, the initial manifestation of injurious MV consists of damage of extra-alveolar vessels leading to interstitial edema, as shown by elevated vWF and cytokine levels in serum but not in BALF. Failure of the endothelial-epithelial barrier occurs at higher stress-strain levels, with alveolar edema, small airway injury, and mechanical alterations. © 2020
Συγγραφείς:
D'Angelo, E.
Koutsoukou, A.
Della Valle, P.
Gentile, G.
Pecchiari, M.
Περιοδικό:
Respiratory Physiology and Neurobiology
Λέξεις-κλειδιά:
albumin; cytokine; von Willebrand factor; cytokine; von Willebrand factor, albumin blood level; animal experiment; animal model; animal tissue; arterial gas; arterial oxygen tension; arterial pressure; Article; artificial ventilation; controlled study; cytokine release; dry weight; lung edema; lung injury; lung lavage; lung mechanics; lung ventilation; male; nonhuman; positive end expiratory pressure; priority journal; protein blood level; rat; tidal volume; adverse event; animal; blood; breathing mechanics; bronchoalveolar lavage fluid; disease model; lung edema; lung injury; pathology; pathophysiology; physiology; Sprague Dawley rat; tidal volume, Animals; Bronchoalveolar Lavage Fluid; Cytokines; Disease Models, Animal; Lung Injury; Male; Pulmonary Edema; Rats; Rats, Sprague-Dawley; Respiration, Artificial; Respiratory Mechanics; Tidal Volume; von Willebrand Factor
DOI:
10.1016/j.resp.2020.103369
