Τίτλος:
Neuronal function of the mRNA decapping complex determines survival of Caenorhabditis elegans at high temperature through temporal regulation of heterochronic gene expression
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
In response to adverse environmental cues, Caenorhabditis elegans larvae can temporarily arrest development at the second moult and form dauers, a diapause stage that allows for long-term survival. This process is largely regulated by certain evolutionarily conserved signal transduction pathways, but it is also affected by miRNA-mediated post-transcriptional control of gene expression. The 5'-3' mRNA decay mechanism contributes to miRNA-mediated silencing of target mRNAs in many organisms but how it affects developmental decisionsduring normalorstressconditionsislargely unknown. Here, we show that loss of the mRNA decapping complex activity acting in the 5'-3' mRNA decay pathway inhibits dauer formationatthestressful high temperature of 27.5°C, and instead promotes early developmental arrest. Our genetic data suggest that this arrest phenotype correlates with dysregulation of heterochronic gene expression and an aberrant stabilization of lin-14 mRNA at early larval stages. Restoration of neuronal dcap-1 activity was sufficient to rescue growth phenotypes of dcap-1 mutants at both high and normal temperatures, implying the involvement of common developmental timing mechanisms. Our work unveils the crucial role of 5'-3' mRNA degradation in proper regulation of heterochronic gene expression programmes, which proved to be essential for survival under stressful conditions. © 2017 The Authors.
Συγγραφείς:
Borbolis, F.
Flessa, C.-M.
Roumelioti, F.
Diallinas, G.
Stravopodis, D.J.
Syntichaki, P.
Περιοδικό:
The Open Biology Journal
Εκδότης:
ROYAL SOCIETY OF LONDON
Λέξεις-κλειδιά:
Caenorhabditis elegans protein; mRNA decapping enzymes; ribonuclease, animal; Caenorhabditis elegans; gene expression regulation; genetics; growth, development and aging; heat; metabolism; mutation; nerve cell; physiological stress; physiology; RNA stability, Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Endoribonucleases; Gene Expression Regulation, Developmental; Hot Temperature; Mutation; Neurons; RNA Stability; Stress, Physiological
