Τίτλος:
Anti-aquaporin-4 autoantibodies in systemic lupus erythematosus persist for years and induce astrocytic cytotoxicity but not CNS disease
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Anti-aquaporin-4 autoantibodies are specific for the neuromyelitis optica spectrum disorders (NMOSD) and they have also been described in patients with systemic lupus erythematosus (SLE) with neurological signs consistent with NMOSD. Our objective was to test for the presence and pathogenicity of anti-AQP4 antibodies in SLE patients without neurological disease. Sera from 89 non-CNS-SLE patients were screened for anti-AQP4 autoantibodies. Two of the 89 patients were positive. Archived samples dating back 11 years were also positive. A brain and spinal cord MRI did not reveal any NMOSD-compatible lesions. An in vitro cytotoxicity assay showed that either sera or purified IgG from these patients induced a complement-mediated damage in cultured astrocytes comparable to antibodies obtained from typical NMO patients. We conclude that AQP4-antibodies can be present in SLE patients and persist for many years, without concurrent clinical or radiological NMOSD signs. It is unclear why the anti-AQP4 antibodies did not induce CNS disease. © 2015 Elsevier B.V..
Συγγραφείς:
Alexopoulos, H.
Kampylafka, E.I.
Fouka, P.
Tatouli, I.
Akrivou, S.
Politis, P.K.
Moutsopoulos, H.M.
Tzioufas, A.G.
Dalakas, M.C.
Περιοδικό:
Journal of Neuroimmunology
Λέξεις-κλειδιά:
aquaporin 4 antibody; complement; immunoglobulin G; AQP4 protein, human; aquaporin 4; autoantibody; enhanced green fluorescent protein; green fluorescent protein, adult; Article; astrocyte; astrocyte culture; cell survival; cell viability; controlled study; cytotoxicity; cytotoxicity assay; female; human; human cell; in vitro study; major clinical study; male; middle aged; myelooptic neuropathy; nuclear magnetic resonance imaging; pathogenicity; priority journal; systemic lupus erythematosus; astrocyte; blood; drug effects; genetic transfection; genetics; HEK293 cell line; immunology; metabolism; nonparametric test; pathology; systemic lupus erythematosus, Aquaporin 4; Astrocytes; Autoantibodies; Female; Green Fluorescent Proteins; HEK293 Cells; Humans; Lupus Erythematosus, Systemic; Male; Statistics, Nonparametric; Transfection
DOI:
10.1016/j.jneuroim.2015.10.007
