Τίτλος:
Corticotropin-releasing hormone exerts direct effects on neuronal progenitor cells: Implications for neuroprotection
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Neurogenesis during embryonic and adult life is tightly regulated by a network of transcriptional, growth and hormonal factors. Emerging evidence indicates that activation of the stress response, via the associated glucocorticoid increase, reduces neurogenesis and contributes to the development of adult diseases.As corticotrophin-releasing hormone (CRH) or factor is the major mediator of adaptive response to stressors, we sought to investigate its involvement in this process. Accordingly, we found that CRH could reverse the damaging effects of glucocorticoid on neural stem/progenitor cells (NS/PCs), while its genetic deficiency results in compromised proliferation and enhanced apoptosis during neurogenesis. Analyses in fetal and adult mouse brain revealed significant expression of CRH receptors in proliferating neuronal progenitors. Furthermore, by using primary cultures of NS/PCs, we characterized the molecular mechanisms and identified CRH receptor-1 as the receptor mediating the neuroprotective effects of CRH. Finally, we demonstrate the expression of CRH receptors in human fetal brain from early gestational age, in areas of active neuronal proliferation. These observations raise the intriguing possibility for CRH-mediated pharmacological applications in diseases characterized by altered neuronal homeostasis, including depression, dementia, neurodegenerative diseases, brain traumas and obesity. © 2013 Macmillan Publishers Limited.
Συγγραφείς:
Koutmani, Y.
Politis, P.K.
Elkouris, M.
Agrogiannis, G.
Kemerli, M.
Patsouris, E.
Remboutsika, E.
Karalis, K.P.
Περιοδικό:
Journal of Molecular Psychiatry
Λέξεις-κλειδιά:
corticotropin releasing factor; corticotropin releasing factor receptor; corticotropin releasing factor receptor 1, animal experiment; apoptosis; article; brain; cell damage; cell proliferation; central nervous system; controlled study; drug mechanism; gestational age; human; human cell; human tissue; immunohistochemistry; in vitro study; in vivo study; mediator; mouse; nerve cell culture; nervous system development; neural stem cell; neuroprotection; nonhuman; priority journal; protein expression; protein function; protein induction, Animals; Apoptosis; Brain; Cell Proliferation; Corticotropin-Releasing Hormone; Dexamethasone; Humans; Mice; Mice, Knockout; Neurogenesis; Neuroprotective Agents; Pyrimidines; Pyrroles; Receptors, Corticotropin-Releasing Hormone; Signal Transduction; Stem Cells
