Τίτλος:
Effective DNA damage response after acute but not chronic immune challenge: SARS-CoV-2 vaccine versus Systemic Lupus Erythematosus
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Whether and how an acute immune challenge may affect DNA Damage Response (DDR) is unknown. By studying vaccinations against Influenza and SARS-CoV-2 (mRNA-based) we found acute increases of type-I interferon-inducible gene expression, oxidative stress and DNA damage accumulation in blood mononuclear cells of 9 healthy controls, coupled with effective anti-SARS-CoV-2 neutralizing antibody production in all. Increased DNA damage after SARS-CoV-2 vaccine, partly due to increased oxidative stress, was transient, whereas the inherent DNA repair capacity was found intact. In contrast, in 26 patients with Systemic Lupus Erythematosus, who served as controls in the context of chronic immune activation, we validated increased DNA damage accumulation, increased type-I interferon-inducible gene expression and induction of oxidative stress, however aberrant DDR was associated with deficiencies in nucleotide excision repair pathways. These results indicate that acute immune challenge can indeed activate DDR pathways, whereas, contrary to chronic immune challenge, successful repair of DNA lesions occurs. © 2021 Elsevier Inc.
Συγγραφείς:
Ntouros, P.A.
Vlachogiannis, N.I.
Pappa, M.
Nezos, A.
Mavragani, C.P.
Tektonidou, M.G.
Souliotis, V.L.
Sfikakis, P.P.
Περιοδικό:
Clinical Immunology Newsletter
Εκδότης:
Academic Press Inc.
Λέξεις-κλειδιά:
bnt 162b 2; comirnaty; complementary DNA; DNA; glyceraldehyde 3 phosphate dehydrogenase; influenza vaccine; kapa sybr fast mastermix; neutralizing antibody; SARS-CoV-2 vaccine; interferon; neutralizing antibody; recombinant vaccine; RNA vaccine, adult; aged; Article; clinical article; controlled study; DNA damage response; female; gene; gene expression; gene induction; housekeeping gene; human; human cell; ifit1 gene; immune response; influenza; innate immunity; male; mx1 gene; nucleotide excision repair; oxidative stress; peripheral blood mononuclear cell; systemic lupus erythematosus; type I interferon signaling; adolescent; case control study; DNA damage; gene expression regulation; immunology; metabolism; middle aged; pathology; physiology; prevention and control; systemic lupus erythematosus; young adult, Adolescent; Adult; Aged; Antibodies, Neutralizing; Case-Control Studies; COVID-19; COVID-19 Vaccines; DNA Damage; Female; Gene Expression Regulation; Humans; Interferon Type I; Lupus Erythematosus, Systemic; Male; Middle Aged; Oxidative Stress; SARS-CoV-2; Vaccines, Synthetic; Young Adult
DOI:
10.1016/j.clim.2021.108765
