Τίτλος:
Sample pooling strategies for SARS-CoV-2 detection
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The worldwide COVID-19 pandemic outburst has caused a serious public health issue with increasing needs of accurate and rapid diagnostic and screening testing. This situation requires an optimized management of the chemical reagents, the consumables, and the human resources, in order to respond accurately and effectively, controlling the spread of the disease. Testing on pooled samples maximizes the number of tested samples, by minimizing the time and the lab supplies needed. The general conceptualization of the pooling method is based on mixing samples together in a batch. Individual testing is needed only if a specific pool exhibits a positive result. The development of alternative hybrid methods, based on “in house” protocols, utilizing commercially available consumables, in combination with a reliable pooling method would provide a solution, focusing on the better exploitation of the personnel and the lab supplies, allowing for rapid screening of a population in a reasonably short time. © 2020 Elsevier B.V.
Συγγραφείς:
Lagopati, N.
Tsioli, P.
Mourkioti, I.
Polyzou, A.
Papaspyropoulos, A.
Zafiropoulos, A.
Evangelou, K.
Sourvinos, G.
Gorgoulis, V.G.
Περιοδικό:
Journal of Virological Methods
Λέξεις-κλειδιά:
clustering algorithm; coronavirus disease 2019; hierarchical clustering; human; mass screening; nonhuman; predictive value; prevalence; priority journal; reliability; reverse transcription polymerase chain reaction; Review; sensitivity and specificity; Severe acute respiratory syndrome coronavirus 2; virus detection; virus load; diagnosis; diagnostic test; epidemiology; isolation and purification; pandemic; procedures; specimen handling, COVID-19; COVID-19 Testing; Diagnostic Tests, Routine; Humans; Pandemics; SARS-CoV-2; Specimen Handling
DOI:
10.1016/j.jviromet.2020.114044
