Τίτλος:
Respiratory infections regulated blood cells IFN-β-PD-L1 pathway in pediatric asthma
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Respiratory infections, in general, and rhinovirus infection specifically are the main reason for asthma exacerbation in children and programmed cell death protein 1 ligand (PD-L1) expression inhibits T cell responses. Objective: Could the interferon (IFN) type I expression in peripheral blood mononuclear cells (PBMCs) improve disease exacerbation in pediatric asthma?. Results: Here we found increased level of PD-L1 messenger RNA (mRNA) in total blood cells isolated from preschool children with virus-induced asthma, with lower percentage of forced expiratory volume in 1 second and with high serum levels of the C-reactive-protein. Conclusions and Clinical Relevance: These data indicate that, in the presence of infection in the airways of preschool children, worse asthma is associated with induced PD-L1 mRNA expression in blood cells. Further, type I IFN, IFN-β, a cytokine that is involved in the clearance of infections, was found to be associated with a better lung function in asthmatic children. These data suggest that improving peripheral blood IFN type I expression in PBMCs in pediatric asthma could improve disease exacerbation due to suppressing PD-L1 expression in blood cells. © 2020 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.
Συγγραφείς:
Kölle, J.
Haag, P.
Vuorinen, T.
Alexander, K.
Rauh, M.
Zimmermann, T.
Papadopoulos, N.G.
Finotto, S.
Περιοδικό:
Immunity, Inflammation and Disease
Λέξεις-κλειδιά:
4 (2 hydroxyethyl) 1 piperazineethanesulfonic acid; allergen; beta interferon; C reactive protein; ficol; glutamine; hypoxanthine phosphoribosyltransferase; interferon; interleukin 10; low density lipoprotein receptor; mercaptoethanol; messenger RNA; nonessential amino acid; penicillin derivative; phytohemagglutinin; programmed death 1 ligand 1; pyruvate sodium; streptomycin; vitamin; beta interferon; CD274 protein, human; ligand; programmed death 1 ligand 1, Article; asthma; atopic dermatitis; bronchoconstriction; cell culture; cell isolation; child; controlled study; density gradient centrifugation; disease exacerbation; disease severity; enzyme linked immunosorbent assay; exhalation; expiratory flow; fetal calf serum; forced expiratory volume; forced vital capacity; gene expression; genetic analysis; human; human cell; immune response; immune system; inflammation; lung function; mRNA expression level; peak expiratory flow; peripheral blood mononuclear cell; phenotype; preschool child; prick test; real time polymerase chain reaction; respiratory tract infection; reverse transcription; Rhinovirus; RNA extraction; RNA isolation; spirometry; turbidimetry; upregulation; virogenesis; virus detection; mononuclear cell, Asthma; B7-H1 Antigen; Child; Child, Preschool; Humans; Interferon-beta; Leukocytes, Mononuclear; Ligands; Respiratory Tract Infections
