Τίτλος:
Epidemiological investigation of Legionella pneumophila serogroup 2 to 14 isolates from water samples by amplified fragment length polymorphism and sequence-based typing and detection of virulence traits
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The aim of this study is to explore the dispersion, clonality, and virulence of Legionella pneumophila serogroups 2 to 14 in the Greek environment. Eighty L. pneumophila serogroup 2 to 14 strains isolated from water distribution systems of hotels, hospitals, athletic venues, and ferries in Greece were tested by monoclonal antibodies (MAbs) for serogroup discrimination and molecularly by amplified fragment length polymorphism (AFLP) for genetic diversity. Fifty-six of 80 strains were also typed by the sequence-based typing (SBT) method. All strains were further analyzed for detection of two pathogenicity loci: Legionella vir homologue (lvh) and repeats in structural toxin (rtxA). Thirty-seven strains (46.2%) belonged to serogroup 6, 26 strains (32.5%) to serogroup 3, and 7 (8.8%) to other serogroups (4, 5, 8, and 10). Ten strains (12.5%) were nontypeable (NT) into the known serogroups. Thirty-nine different AFLP types were found among the 80 L. pneumophila serogroup 2 to 14 strains, and 24 different SBT types were found among the 56 strains tested. Among the 80 strains, the lvh locus was present in 75 (93.8%), the rtxA locus was found in 76 (95%), and both loci were found in 73 (91.3%) strains. This study showed that there is genetic variability of L. pneumophila serogroups 2 to 14 in the Greek environment as well as a high percentage of the pathogenicity loci. Introducing an effective diagnostic test for L. pneumophila serogroups 2 to 14 in urine and promoting the examination of respiratory specimens from patients hospitalized for pneumonia in Greek hospitals are essential. © 2016, American Society for Microbiology. All Rights Reserved.
Συγγραφείς:
Katsiaflaka, A.
Pournaras, S.
Kristo, I.
Mouchtouri, V.A.
Kyritsi, M.
Velonakis, E.
Vatopoulos, A.C.
Hadjichristodoulou, C.
Περιοδικό:
Applied and Environmental Microbiology
Εκδότης:
American Society for Microbiology
Λέξεις-κλειδιά:
Diagnosis; Hospitals; Monoclonal antibodies, Amplified fragment length polymorphisms; Diagnostic tests; Genetic diversity; Genetic variability; Legionella; Legionella pneumophila; Monoclonal antibodies (mAbs); Water samples, Water distribution systems, antibody; bacterium; epidemiology; genetic analysis; genetic marker; genetic variation; pathogenicity; toxin; virulence, Greece, Legionella; Legionella pneumophila; Legionella pneumophila serogroup 2; Vir, fresh water, amplified fragment length polymorphism; bacterium identification; classification; genetics; Greece; human; isolation and purification; Legionella pneumophila; Legionnaires' Disease; microbiology; water pollution, Amplified Fragment Length Polymorphism Analysis; Bacterial Typing Techniques; Fresh Water; Greece; Humans; Legionella pneumophila; Legionnaires' Disease; Water Pollution
DOI:
10.1128/AEM.01672-16
