Τίτλος:
Hepatitis B-specific T helper cell responses in uninfected infants born to HBsAg+/HBeAg- mothers
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Vertically transmitted hepatitis B virus (HBV) usually causes chronic infection. While combined active-passive immunoprophylaxis in neonates of hepatitis B surface antigen-positive (HBsAg ) mothers at birth prevents vertical transmission, it is not yet clear whether neonates encounter the virus or its products in the absence of hepatitis B e antigen (HBeAg). This study was undertaken to investigate HBV antigen-specific T-cell responses in vaccinated neonates of HBsAg+/HBeAg- mothers. Blood was collected from 46 HBsAg mothers and their neonates (subjects) as well as 24 age-matched controls. All neonates of HBsAg mothers received appropriate immunoprophylaxis, and HBsAg and hepatitis B surface antibody (anti-HBs) antibody titers were determined after completion of the vaccination course. Peripheral blood mononuclear cells (PBMCs) from infants at birth, 1 and 6 months of age were stimulated with recombinant HBsAg, hepatitis B core antigen (HBcAg) and mitogen, and interferon (IFN)-γ concentrations were determined by ELISA. HBsAg-induced production of IL-2, IL-5, IL-6 and IL-10 was assessed using a cytometric bead array kit on cells from 6-month-old neonates post-vaccination. All neonates were HBsAg and responded to vaccination. Increased IFN-γ production following HBcAg stimulation was seen in 30.4% of neonates born to HBsAg+/HBeAg- mothers. Subjects demonstrated significantly higher IL-2 production post-HBsAg stimulation, whereas IL-5, IL-6 and IL-10 cytokine responses were not significantly different. Almost one-third of uninfected neonates developed viral antigen-induced IFN-γ production, suggesting that they had been exposed to virions or viral derivatives. This encounter, however, did not impair their T-cell responses to vaccination. © 2010 CSI and USTC. All rights reserved.
Συγγραφείς:
Koumbi, L.
Bertoletti, A.
Anastasiadou, V.
MacHaira, M.
Goh, W.
Papadopoulos, N.G.
Kafetzis, D.A.
Papaevangelou, V.
Περιοδικό:
Cellular and Molecular Immunology
Λέξεις-κλειδιά:
gamma interferon; hepatitis B antibody; hepatitis B core antigen; hepatitis B surface antigen; hepatitis B(e) antigen; interleukin 10; interleukin 2; interleukin 5; interleukin 6; mitogenic agent; recombinant hepatitis B vaccine; virus antigen, antibody titer; antigen specificity; article; controlled study; cytokine production; cytokine release; enzyme linked immunosorbent assay; female; flow cytometry; hepatitis B; human; human cell; immunoprophylaxis; immunostimulation; infant; infection prevention; newborn; peripheral blood mononuclear cell; post exposure prophylaxis; T lymphocyte activation; Th1 cell; Th2 cell; treatment response; virion; virus load, Case-Control Studies; Female; Hepatitis B; Hepatitis B e Antigens; Hepatitis B Surface Antigens; Humans; Infant, Newborn; Interferon-gamma; Pregnancy; T-Lymphocytes, Helper-Inducer, Hepatitis B virus
