Τίτλος:
Interferon (IFN)-stimulated gene 15: A novel biomarker for lymphoma development in Sjögren's syndrome
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objective: We investigated whether interferon (IFN) induced genes could serve as biomarkers for the detection of lymphoma development among patients with Sjögren's syndrome (SS). Methods: Total RNA was extracted from 98 labial minor salivary glands (LMSG) biopsies of SS patients [61 not complicated by lymphoma (SS-nL) and 37 complicated by Non-Hodgkin Lymphoma (NHL) (SS-L)] and 67 matched peripheral blood (PB) samples, as well as from 30 LMSG biopsies and 17 matched PB derived from sicca controls (SC). RNA sequencing was performed in LMSG biopsies of high and low risk SS patients for lymphoma development and SC. Expression analysis of type I (MX-1, IFIT-1, IFI44 and ISG-15) and type II IFN induced (CXCL9/MIG-1, GBP-1) genes was performed by real time PCR. Results: ISG-15 transcript levels were significantly higher in SS-L patients compared to SS-nL patients in both LMSG tissues and PB specimens. Additionally, MIG-1 was found to display higher expression values in LMSG tissues, but not in PB derived from SS-L patients compared to the SS-nL group. A coordinate expression in PB/LMSG of type I IFN (ISG-15, MX-1 and IFI44), but not type II IFN induced genes was also observed. Conclusion: ISG-15 gene expression was able to distinguish SS-nL and SS-L at both periphery and tissue level and therefore could represent a novel biomarker for lymphoma development among SS patients. PB and LSMG seem to share a common transcriptional profile of type I IFN pathway. © 2021 Elsevier Ltd
Συγγραφείς:
Cinoku, I.I.
Verrou, K.-M.
Piperi, E.
Voulgarelis, M.
Moutsopoulos, H.M.
Mavragani, C.P.
Περιοδικό:
Journal of Autoimmune Diseases
Εκδότης:
INSTAP Academic Press
Λέξεις-κλειδιά:
biological marker; CXCL9 chemokine; gamma interferon; glyceraldehyde 3 phosphate dehydrogenase; guanylate binding protein 1; interferon; interferon induced protein 44; interferon induced protein with tetratricopeptide repeat 1; messenger RNA; myxovirus resistance 1 protein; unclassified drug; biological marker; cytokine; interferon; ISG15 protein, human; messenger RNA; ubiquitin, adult; Article; biopsy; blood; blood sampling; cancer growth; controlled study; diagnostic test accuracy study; diffuse large B cell lymphoma; female; gene expression; gene expression level; gene expression profiling; genetic marker; histopathology; human; human tissue; interferon stimulated gene 15; lymphocytic lymphoma; major clinical study; male; marginal zone lymphoma; marker gene; minor saliva gland; mRNA expression level; nodal marginal zone lymphoma; nonhodgkin lymphoma; real time polymerase chain reaction; RNA extraction; RNA sequencing; salivary gland; Sjoegren syndrome; T cell lymphoma; transcriptomics; undifferentiated connective tissue disease; aged; complication; genetics; immunology; lymphoma; middle aged; pathology; physiology; Sjoegren syndrome, Adult; Aged; Biomarkers; Cytokines; Female; Humans; Interferon Type I; Lymphoma; Male; Middle Aged; RNA, Messenger; Sjogren's Syndrome; Ubiquitins
DOI:
10.1016/j.jaut.2021.102704
