Τίτλος:
Dissemination of International Clone II Acinetobacter baumannii Strains Coproducing OXA-23 Carbapenemase and 16S rRNA Methylase ArmA in Athens, Greece
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The aim of this study was to study the molecular epidemiology of 16S rRNA-methylase (RMT)-producing clinical Acinetobacter baumannii isolates from hospitals in Athens, Greece. Single-patient A. baumannii clinical isolates, coresistant to amikacin and gentamicin (n = 347), from five tertiary care hospitals, were submitted to minimum inhibitory concentration determination and molecular testing for carbapenemase and RMT genes. A. baumannii, resistant to amikacin and gentamicin, was isolated at participating institutions at a mean rate of 67.8%. Among them 93.7% harbored the armA. The vast majority (98.5%) of armA positive isolates were OXA-23 producers, assigned mainly (99.4%) to sequence group G1, corresponding to international clone (IC) II. Four isolates (all from the same hospital) were OXA-24 producers (1.2%), assigned to G6 corresponding to CC78 and only one isolate was OXA-58-producer, assigned to G2 (IC I). Apramycin was the most active agent inhibiting 99.7% of the isolates at ≤64 mg/L, whereas colistin, trimethoprim/sulfamethoxazole, minocycline, and tigecycline exhibited only sparse activity (S, <18%). RMT production is an emerging mechanism of resistance, capable of compromising the clinical efficacy of aminoglycosides. High prevalence of armA was observed among A. baumannii strains isolated in participating hospitals in Athens, which were mainly OXA-23 producers and belonged to IC II. Apramycin is a structurally unique aminoglycoside, currently used as a veterinary agent. Although it has not been evaluated for clinical use, apramycin appears worthy of further investigation for repurposing as a human therapeutic against difficult-to-treat pathogens. © Copyright 2020, Mary Ann Liebert, Inc., publishers 2020.
Συγγραφείς:
Nafplioti, K.
Galani, I.
Angelidis, E.
Adamou, P.
Moraitou, E.
Giannopoulou, P.
Chra, P.
Damala, M.
Vogiatzakis, E.
Trikka-Graphakos, E.
Baka, V.
Prifti, E.
Antoniadou, A.
Souli, M.
Περιοδικό:
Microbial Drug Resistance: Mechanism, Epidemiology, and Disease
Εκδότης:
Mary Ann Liebert, Inc. 140 Huguenot Street, 3rd Floor New Rochelle, NY 10801 USA
Λέξεις-κλειδιά:
amikacin; aminoglycoside; apramycin; carbapenemase; colistin; cotrimoxazole; gentamicin; methyltransferase; minocycline; RNA 16S; tigecycline; tobramycin; amikacin; aminoglycoside; antiinfective agent; beta lactamase; beta-lactamase OXA-23; gentamicin; RNA 16S; transfer RNA methyltransferase, Acinetobacter baumannii; antibiotic sensitivity; Article; bacterium isolate; enzyme synthesis; Greece; minimum inhibitory concentration; molecular epidemiology; nonhuman; priority journal; Acinetobacter baumannii; Acinetobacter infection; clinical trial; drug effect; genetics; human; isolation and purification; microbial sensitivity test; microbiology; multicenter study; multidrug resistance, Acinetobacter baumannii; Acinetobacter Infections; Amikacin; Aminoglycosides; Anti-Bacterial Agents; beta-Lactamases; Drug Resistance, Multiple, Bacterial; Gentamicins; Greece; Humans; Microbial Sensitivity Tests; Molecular Epidemiology; RNA, Ribosomal, 16S; tRNA Methyltransferases
DOI:
10.1089/mdr.2019.0075
