Τίτλος:
Differential expression patterns of Toll Like Receptors and Interleukin-37 between calcific aortic and mitral valve cusps in humans
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Significant differences are mentioned in the progress of calcification between aortic and mitral valve. Evidence of inflammation in calcific aortic and mitral valve disease suggests that pathways of Toll Like Receptors (TLR) and Interleukin (IL)-37 expression may contribute to this process. We sought to investigate the role of TLR-mediated inflammatory response and IL-37 pathway expression on aortic and mitral valve calcification. Material and methods: One-hundred twenty stenotic valve cusps/leaflets (60 aortic, 60 mitral) were excised during surgery and were collected for histological, immunohistochemistry and morphometric analysis at our department. After total RNA isolation from a second part of valve cusps/leaflets, cDNA synthesis and quantitative reverse transcription polymerase chain reaction (qRT-PCR) protocols were performed and relative mRNA levels of target genes were assessed. Results: By histological analysis, the anti-inflammatory IL-37 levels were increased in mitral valve leaflets (MVL) compared to aortic valve cusps (AVCu) while all other biomarkers, including TLR, presented a reverse pattern with decreased levels as compared to AVCu. In terms of calcification biomarkers, only osteopontin differed between AVCu and MVL. mRNA analysis confirmed increased expression of IL-37 and decreased levels of TLR in MVL compared to AVCu. Conclusions: Stenotic cusps of aortic valves express lower IL-37 and increased TLRs levels than stenotic mitral valve leaflets, suggesting a differential pro-calcification and pro-inflammatory profile between the two valves. This may explain the higher incidence of calcification of AVCu than MVL and offer therapeutic considerations. © 2019 Elsevier Ltd
Συγγραφείς:
Kapelouzou, A.
Kontogiannis, C.
Tsilimigras, D.I.
Georgiopoulos, G.
Kaklamanis, L.
Tsourelis, L.
Cokkinos, D.V.
Εκδότης:
INSTAP Academic Press
Λέξεις-κλειδιά:
biological marker; complementary DNA; interleukin 37; messenger RNA; osteopontin; toll like receptor; biological marker; cytokine; IL37 protein, human; interleukin 1; messenger RNA; toll like receptor, aged; aortic valve; aortic valve stenosis; Article; calcification; controlled study; female; gene expression; histology; human; human tissue; immunohistochemistry; inflammation; male; mitral valve; mitral valve stenosis; morphometry; mRNA expression level; priority journal; protein expression; quantitative analysis; reverse transcription polymerase chain reaction; RNA analysis; RNA isolation; signal transduction; aortic valve; calcinosis; cardiomyopathy; genetics; metabolism; mitral valve; pathology; valvular heart disease, Aged; Aortic Valve; Biomarkers; Calcinosis; Cardiomyopathies; Cytokines; Female; Heart Valve Diseases; Humans; Inflammation; Interleukin-1; Male; Mitral Valve; RNA, Messenger; Toll-Like Receptors
DOI:
10.1016/j.cyto.2019.01.009
