Τίτλος:
Identification of the zinc, copper and cadmium metalloproteome of the protozoon Tetrahymena thermophila by systematic bioinformatics
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Tetrahymena thermophila (T. thermophila) is a ciliated protozoon that can detect freshwater pollution by heavy metals (“whole-cell biosensor”). This work employed a systematic bioinformatics approach to predict and analyze the metalloproteome of T. thermophila for the essential Zn, Cu and the non-essential Cd. 3784 metal-binding proteins were identified compared to the 456 annotated so far in UniProt. The localization, functional classification, and the functionally enriched network of the newly identified metalloproteome are presented. Cd toxicity could be explained in terms of the metal replacing Cu and especially Zn in MAPKs, transporters and antioxidant enzymes. The predicted results for Cd toxicity and responses reflect those observed experimentally in different organisms after their exposure to Cd. © 2017, Springer-Verlag Berlin Heidelberg.
Συγγραφείς:
Chasapis, C.T.
Andreini, C.
Georgiopolou, A.K.
Stefanidou, M.E.
Vlamis-Gardikas, A.
Περιοδικό:
Archives of Microbiology
Λέξεις-κλειδιά:
cadmium binding protein; copper protein; metalloprotein; protozoal protein; zinc binding protein; antioxidant; cadmium; cadmium-binding protein; carrier protein; copper; copper-binding protein; fresh water; metalloprotein; metallothionein; zinc; zinc-binding protein, Article; cell death; genome analysis; ion transport; membrane transport; metal binding; mitosis; molecular genetics; nitrogen metabolism; nonhuman; oxidative stress; priority journal; protein analysis; protein function; protein localization; protein modification; protein phosphorylation; protein structure; proteomics; protozoal genetics; signal transduction; Tetrahymena thermophila; type strain; animal; biology; chemistry; metabolism; parasitology; Tetrahymena thermophila; water pollutant; water pollution, Animals; Antioxidants; Cadmium; Carrier Proteins; Computational Biology; Copper; Fresh Water; Metalloproteins; Metallothionein; Tetrahymena thermophila; Water Pollutants, Chemical; Water Pollution; Zinc
DOI:
10.1007/s00203-017-1385-y
