Τίτλος:
Analysis of the cell populations composing the mononuclear cell infiltrates in the labial minor salivary glands from patients with rheumatoid arthritis and sicca syndrome
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objectives Sicca symptoms occur in around 30% of rheumatoid arthritis (RA) patients. Herein, we examined the characteristics of RA patients bearing sicca symptomatology (RA-sicca) with a special focus on the immunohistopathological features of their labial minor salivary gland (LMSG) biopsies. Methods Our cohort included 100 consecutive RA patients which were interrogated using a sicca symptoms questionnaire. Positive responders were evaluated for ocular and oral dryness and underwent an LMSG biopsy. All samples were immunohistochemically evaluated for the presence and distribution of specific leukocyte subsets using appropriate markers and for the expression of certain immunoregulatory molecules by salivary gland epithelial cells. Positively stained and total mononuclear cells (MNC) were counted in the entire section. Counts were expressed as cell frequency (percentage of cell type number/total infiltrating MNC number). Results In the majority (86.1%) of the 44 RA-sicca cases, periductal infiltrates were observed in LMSG biopsies. The frequencies of infiltrating cell subtypes and their correlation with lesion severity were different from that previously described in primary Sjögren's syndrome (pSS). Moreover, DCs and ΜΦs frequencies were increased in RA-sicca patients who had a biopsy focus score <1 and absence of anti-Ro/anti-La autoantibodies, in contrast to what was observed for B cells. In about half of the biopsies, salivary gland epithelial cells expressed CD80/B7.1 molecules, most commonly in patients with a positive biopsy or anti-Ro/anti-La autoantibodies. Conclusion LMSG infiltrates composition in RA-sicca patients is distinct from that described in pSS. These differences, further attest to diverse pathophysiologic processes operating in these two entities. © 2016
Συγγραφείς:
Fragoulis, G.E.
Fragkioudaki, S.
Reilly, J.H.
Kerr, S.C.
McInnes, I.B.
Moutsopoulos, H.M.
Περιοδικό:
Journal of Autoimmune Diseases
Εκδότης:
INSTAP Academic Press
Λέξεις-κλειδιά:
CD8 antigen; HLA DR antigen; autoantibody; autoantigen; B7 antigen; ribonucleoprotein; SS-A antigen; SS-B antigen, adult; aged; antigen expression; antigen presenting cell; Article; B lymphocyte; CD4 CD8 ratio; cell composition; cell infiltration; cell population; cell subpopulation; cohort analysis; eye disease; female; histopathology; human; human tissue; labial minor salivary gland; major clinical study; male; minor saliva gland; mononuclear cell; ocular dryness; priority journal; rheumatoid arthritis; salivary gland biopsy; sicca symptoms questionnaire; Sjoegren syndrome; xerostomia; biopsy; cytology; dendritic cell; immunohistochemistry; immunology; lip; macrophage; metabolism; middle aged; minor saliva gland; pathology; questionnaire; retrospective study; rheumatoid arthritis; Sjoegren syndrome; T lymphocyte; very elderly; young adult, Adult; Aged; Aged, 80 and over; Arthritis, Rheumatoid; Autoantibodies; Autoantigens; B-Lymphocytes; B7-1 Antigen; Biopsy; Dendritic Cells; Female; Humans; Immunohistochemistry; Lip; Macrophages; Male; Middle Aged; Retrospective Studies; Ribonucleoproteins; Salivary Glands, Minor; Sjogren's Syndrome; Surveys and Questionnaires; T-Lymphocytes; Young Adult
DOI:
10.1016/j.jaut.2016.06.008
