Τίτλος:
Adrenergic Effect on Cytokine Release After Ex Vivo Healthy Volunteers’ Whole Blood LPS Stimulation
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Catecholamines are molecules with immunomodulatory properties in health and disease. Several studies showed the effect of catecholamines when administered to restore hemodynamic stability in septic patients. This study investigates the effect of norepinephrine and dobutamine on whole blood cytokine release after ex vivo lipopolysaccharide (LPS) stimulation. Whole blood collected from healthy individuals was stimulated with LPS, in the presence of norepinephrine or dobutamine at different concentrations, with or without metoprolol, a β1 receptor antagonist. Cytokine measurement was performed in isolated cell culture supernatants with ELISA. Results are expressed as mean ± SEM and compared with Mann-Whitney rank-sum test. Both norepinephrine and dobutamine significantly reduced TNF-α and IL-6 production after ex vivo LPS stimulation of whole blood in a dose-dependent manner, and this effect was partially reversed by the presence of metoprolol. Norepinephrine and dobutamine reduce the LPS-induced production of pro-inflammatory cytokines, thus possibly contributing to altered balance between the inflammatory and anti-inflammatory responses, which are vital for a successful host response to severe disease, shock, and sepsis. © 2016, Springer Science+Business Media New York.
Συγγραφείς:
Papandreou, V.
Kavrochorianou, N.
Katsoulas, T.
Myrianthefs, P.
Venetsanou, K.
Baltopoulos, G.
Περιοδικό:
Journal of Inflammation Research
Εκδότης:
Springer New York LLC
Λέξεις-κλειδιά:
dobutamine; interleukin 6; lipopolysaccharide; metoprolol; noradrenalin; tumor necrosis factor alpha; adrenergic receptor stimulating agent; cytokine; dobutamine; interleukin 6; lipopolysaccharide; noradrenalin; tumor necrosis factor, adrenergic activity; adult; Article; cytokine production; cytokine release; enzyme linked immunosorbent assay; ex vivo study; human; male; normal human; scanning electron microscopy; biosynthesis; blood; dose response; drug effects; metabolism; sepsis, Adrenergic Agents; Blood; Cytokines; Dobutamine; Dose-Response Relationship, Drug; Healthy Volunteers; Humans; Interleukin-6; Lipopolysaccharides; Norepinephrine; Sepsis; Tumor Necrosis Factor-alpha
DOI:
10.1007/s10753-016-0338-y
