Τίτλος:
Adsorption of major endoglucanase from Thermoascus aurantiacus on cellulosic substrates
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A thermostable endoglucanase (EndoI) was produced by the thermophilic fungus Thermoascus aurantiacus when grown on cellulosic materials under submerged culture (SC) and solid-state fermentation (SSF). In both cultivation techniques a considerable amount of enzyme activity remained adsorbed onto solid particles, and this was taken into consideration when modeling enzyme production. The results were compatible with the assumption that, following its synthesis, an amount of EndoI was bound on substrate and gradually released into the liquid medium. Adsorption of the enzyme on crystalline cellulose was confirmed in vitro by experiments with purified endoglucanase, which was isolated by anion exchange chromatography. The Langmuir isotherm could efficiently describe the adsorption kinetics, and the estimated A max and K ad values compared with those obtained for cellulases bearing a binding domain. EndoI displayed high affinity for crystalline cellulose and low binding capacity, which could be beneficial in textile processing. © 2009 Springer Science+Business Media B.V.
Συγγραφείς:
Mamma, D.
Hatzinikolaou, D.
Kekos, D.
Stamatis, H.
Kalogeris, E.
Περιοδικό:
World Journal of Microbiology and Biotechnology
Λέξεις-κλειδιά:
Adsorption kinetics; Anion-exchange chromatographies; Binding capacities; Binding domains; Cellulosic materials; Cellulosic substrates; Crystalline cellulose; Endoglucanase; Enzyme productions; High affinities; In-vitro; Langmuir isotherms; Liquid mediums; Solid particles; Solid-state fermentations; Submerged cultures; Thermoascus aurantiacus; Thermophilic fungus, Adsorption; Biochemical engineering; Cellulose; Chromatographic analysis; Crystalline materials; Enzyme activity; Enzymes; Fermentation; Substrates, Textile processing, Adsorption; Biochemistry; Chromatography; Cultivation; Fermentation; Glucanase; Substrates, Fungi; Thermoascus; Thermoascus aurantiacus
DOI:
10.1007/s11274-008-9949-2
