Τίτλος:
Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Aspergillus pathogens usually infect immunocompromised patients with lethal outcome. We report a multiplex PCR assay for the discrimination of the most frequent Aspergillus pathogens, A. fumigatus, A. flavus, A. niger and A. terreus, through distinct amplicons of 250 bp, 200 bp, 150 bp and 450 bp respectively, derived from the rDNA gene of A. terreus and the aspergillopepsin genes of the remaining species. © 2008 Elsevier B.V. All rights reserved.
Συγγραφείς:
Logotheti, M.
Kotsovili-Tseleni, A.
Arsenis, G.
Legakis, N.I.
Περιοδικό:
Journal of Microbiological Methods
Λέξεις-κλειδιά:
aspergillopepsin; pepsin A; ribosome DNA; unclassified drug, amplicon; animal experiment; article; Aspergillus flavus; Aspergillus fumigatus; Aspergillus niger; Aspergillus terreus; discriminant analysis; fungal strain; gene sequence; human; human experiment; multiplex polymerase chain reaction; nonhuman; normal human; pathogenesis; priority journal, Aspergillosis; Aspergillus flavus; Aspergillus fumigatus; Aspergillus niger; DNA Primers; DNA, Fungal; Humans; Mycological Typing Techniques; Polymerase Chain Reaction; Sensitivity and Specificity, Aspergillus; Aspergillus flavus; Aspergillus fumigatus; Aspergillus niger; Aspergillus terreus
DOI:
10.1016/j.mimet.2008.10.006
