Τίτλος:
Rhinovirus infection and house dust mite exposure synergize in inducing bronchial epithelial cell interleukin-8 release
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Human rhinoviruses (HRVs) and house dust mites (HDMs) are among the most common environmental factors able to induce airway inflammation in asthma. Although epidemiological studies suggest that they also synergize in inducing asthma exacerbations, there is no experimental evidence to support this, nor any information on the possible mechanisms involved. Objective: To investigate their interaction on the induction of airway epithelial inflammatory responses in vitro. Methods: BEAS-2B cells were exposed to activated HDM Dermatophagoides pteronyssinus major allergen I (Der p I), HRVs (HRV1b or HRV16) or both in different sequences. IL-8/CXCL8 release, intercellular adhesion molecule (ICAM)-1 surface expression and nuclear factor κB (NF-κB) translocation were evaluated. Complementary, primary human bronchial epithelial cells (HBECs) exposed to both Der p I and RVs and IL-8, IL-6, IFN-γ-induced protein (IP)-10/CXCL10, IFN-λ1/IL-29, regulated upon activation normal T lymphocyte expressed and secreted (RANTES)/CCL5 release were measured. Results: RV and Der p I up-regulated IL-8 release, ICAM-1 expression and NF-κB translocation in BEAS-2B cells. Simultaneous exposure to both factors, as well as when cells were initially exposed to HRV and then to Der p I, resulted in further induction of IL-8 in a synergistic manner. Synergism was not observed when cells were initially exposed to Der p I and then to HRV. This was the pattern in ICAM-1 induction although the phenomenon was not synergistic. Concurrent exposure induced an early synergistic NF-κB translocation induction, differentiating with time, partly explaining the above observation. In HBECs, both HRV and Der p I induced IL-8, IL-6, IL-29 and IP-10, while RANTES was induced only by HRV. Synergistic induction was observed only in IL-8. Conclusion: HRV and enzymatically active Der p I can act synergistically in the induction of bronchial epithelial IL-8 release, when HRV infection precedes or is concurrent with Der p I exposure. Such a synergy may represent an important mechanism in virus-induced asthma exacerbations. © 2008 The Authors.
Συγγραφείς:
Bossios, A.
Gourgiotis, D.
Skevaki, C.L.
Saxoni-Papageorgiou, P.
Lötvall, J.
Psarras, S.
Karpathios, T.
Constandopoulos, A.G.
Johnston, S.L.
Papadopoulos, N.G.
Περιοδικό:
Clinical and Experimental Allergy
Λέξεις-κλειδιά:
dermatophagoides pteronyssinus major allergen I; gamma interferon inducible protein 10; house dust allergen; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; interleukin 29; interleukin 6; interleukin 8; RANTES; unclassified drug, article; asthma; bronchus mucosa; controlled study; disease exacerbation; dust exposure; house dust; human; human cell; in vitro study; priority journal; protein expression; protein secretion; respiratory tract inflammation; Rhinovirus, Animals; Antigens, Dermatophagoides; Cell Adhesion Molecules; Cell Line; Cytokines; Epithelial Cells; Humans; Interleukin-8; Picornaviridae Infections; Protein-Serine-Threonine Kinases; Pyroglyphidae; Rhinovirus; Virus Replication
DOI:
10.1111/j.1365-2222.2008.03058.x
