Τίτλος:
Molecular phylogeny of VP1, 2A, and 2B genes of echovirus isolates: Epidemiological linkage and observations on genetic variation
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Phylogenetic relationships between 37 echovirus clinical isolates, most of them originating from an aseptic meningitis outbreak during 2001 in Greece, were investigated by RT-PCR and sequencing. The generic primers 292 and 222 were used to amplify about 300 bp of the 5′ end of VP1 while primers EUG3a, 3b, 3c, and EUC2 amplified the entire coding sequence of the 2A and 2B genes. Phylogenetic trees were constructed for each genomic region using the clinical isolates' sequences and those of the prototype echoviruses in order to investigate the correlation of part of VP1 with the serotype as well as the genetic variation of the echovirus genome in 2A and 2B. The phylogenetic grouping pattern of the clinical isolates revealed that there is a correlation of serotype and genotype in the part of VP1 that was investigated, while this pattern is disrupted in the adjacent genomic regions that were sequenced. Sequence analysis of the adjacent 2A and 2B genes provided a different pattern of phylogenetic relationships and strong evidence of epidemiological linkage of most of the clinical isolates. © Springer-Verlag 2006.
Συγγραφείς:
Kottaridi, C.
Bolanaki, E.
Mamuris, Z.
Stathopoulos, C.
Markoulatos, P.
Περιοδικό:
Archives of Virology
Λέξεις-κλειδιά:
capsid protein; virus protein; virus RNA, amino acid sequence; article; aseptic meningitis; classification; DNA sequence; Echo virus; Enterovirus; epidemic; epidemiology; genetic variability; genetics; Greece; human; isolation and purification; molecular genetics; nucleotide sequence; phylogeny; reverse transcription polymerase chain reaction; serotyping; virology; virus gene; virus infection, Amino Acid Sequence; Capsid Proteins; Disease Outbreaks; Echovirus 6, Human; Echovirus Infections; Enterovirus B, Human; Epidemiology, Molecular; Genes, Viral; Greece; Humans; Meningitis, Aseptic; Molecular Sequence Data; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Sequence Analysis, DNA; Serotyping; Variation (Genetics); Viral Nonstructural Proteins, Echovirus
DOI:
10.1007/s00705-005-0697-4
