Τίτλος:
Detection of ochratoxin A in beer samples with a label-free monolithically integrated optoelectronic biosensor
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
An optical biosensor for label-free detection of ochratoxin A (OTA) in beer samples is presented. The biosensor consists of an array of ten Mach-Zehnder interferometers (MZIs) monolithically integrated along with their respective broad-band silicon light sources on the same Si chip (37 mm2). The chip was transformed to biosensor by functionalizing the MZIs sensing arms with an OTA-ovalbumin conjugate. OTA determination was performed by pumping over the chip mixtures of calibrators or samples with anti-OTA antibody following a competitive immunoassay format. An external miniaturized spectrometer was employed to continuously record the transmission spectra of each interferometer. Spectral shifts obtained due to immunoreaction were transformed to phase shifts through Discrete Fourier Transform. The assay had a detection limit of 2.0 ng/ml and a dynamic range 4.0–100 ng/ml in beer samples, recoveries ranging from 90.6 to 116%, and intra- and inter-assay coefficients of variation of 9% and 14%, respectively. The results obtained with the sensor using OTA-spiked beer samples spiked were in good agreement with those obtained by an ELISA developed using the same antibody. The good analytical performance of the biosensor and the small size of the proposed chip provide for the development of a portable instrument for point-of-need determinations. © 2016 Elsevier B.V.
Συγγραφείς:
Pagkali, V.
Petrou, P.S.
Salapatas, A.
Makarona, E.
Peters, J.
Haasnoot, W.
Jobst, G.
Economou, A.
Misiakos, K.
Raptis, I.
Kakabakos, S.E.
Περιοδικό:
Journal of Hazardous Materials
Λέξεις-κλειδιά:
Antibodies; Biosensors; Discrete Fourier transforms; Integrated optoelectronics; Light sources; Mach-Zehnder interferometers; Monolithic integrated circuits, Analytical performance; Competitive immunoassay; Inter-assay coefficients; Label-free detection; Monolithically integrated; Ochratoxin A; Silicon light sources; Transmission spectrums, Beer, immunoglobulin G antibody; ochratoxin; toxin antibody; ochratoxin; silicon, antibody; assay; detection method; electronic equipment; immunoassay; interferometer; performance assessment; sensor; spectral analysis; toxin; wine, antibody blood level; Article; beer; calibrator; controlled study; enzyme immunoassay; enzyme linked immunosorbent assay; interferometer; limit of detection; liquid chromatography; miniaturization; optical biosensor; process optimization; reaction time; refraction index; tandem mass spectrometry; analysis; beer; chemistry; devices; food contamination; genetic procedures; immunoassay; interferometry; light related phenomena; limit of detection; procedures, Beer; Biosensing Techniques; Food Contamination; Immunoassay; Interferometry; Limit of Detection; Ochratoxins; Optical Phenomena; Silicon
DOI:
10.1016/j.jhazmat.2016.03.019
