Τίτλος:
Prognostic role of APC and RASSF1A promoter methylation status in cell free circulating DNA of operable gastric cancer patients
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Gastric carcinogenesis is a multistep process including not only genetic mutations but also epigenetic alterations. The best known and more frequent epigenetic alteration is DNA methylation affecting tumor suppressor genes that may be involved in various carcinogenetic pathways. The aim of the present study was to investigate the methylation status of APC promoter 1A and RASSF1A promoter in cell free DNA of operable gastric cancer patients.Using methylation specific PCR, we examined the methylation status of APC promoter 1A and RASSF1A promoter in 73 blood samples obtained from patients with gastric cancer.APC and RASSF1A promoters were found to be methylated in 61 (83.6%) and 50 (68.5%) of the 73 gastric cancer samples examined, but in none of the healthy control samples ( p<. 0.001). A significant association between methylated RASSF1A promoter status and lymph node positivity was observed ( p= 0.005). Additionally, a significant correlation between a methylated APC promoter and elevated CEA ( p= 0.033) as well as CA-19.9 ( p= 0.032) levels, was noticed. The Kaplan-Meier estimates of survival, significantly favored patients with a non-methylated APC promoter status ( p= 0.008). No other significant correlations between APC and RASSF1A methylation status and different tumor variables examined was observed.Serum RASSF1A and APC promoter hypermethylation is a frequent epigenetic event in patients with early operable gastric cancer. The observed correlations between APC promoter methylation status and survival as well as between a hypermethylated RASSF1A promoter and nodal positivity may be indicative of a prognostic role for those genes in early operable gastric cancer. Additional studies, in a larger cohort of patients are required to further explore whether these findings could serve as potential molecular biomarkers of survival and/or response to specific treatments. © 2015 Elsevier B.V.
Συγγραφείς:
Balgkouranidou, I.
Matthaios, D.
Karayiannakis, A.
Bolanaki, H.
Michailidis, P.
Xenidis, N.
Amarantidis, K.
Chelis, L.
Trypsianis, G.
Chatzaki, E.
Lianidou, E.S.
Kakolyris, S.
Περιοδικό:
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
Λέξεις-κλειδιά:
DNA; CA 19-9 antigen; carcinoembryonic antigen; DNA; RASSF1 protein, human; tumor marker; tumor suppressor protein, adult; aged; APC gene; Article; cancer prognosis; controlled study; correlational study; DNA methylation; epigenetics; female; gene; human; major clinical study; overall survival; polymerase chain reaction; priority journal; promoter region; RASSF1A gene; stomach cancer; survival rate; blood; carcinoma; comparative study; genetics; Kaplan Meier method; lymph node metastasis; male; middle aged; mortality; proportional hazards model; secondary; Stomach Neoplasms; tumor suppressor gene; very elderly, Adult; Aged; Aged, 80 and over; CA-19-9 Antigen; Carcinoembryonic Antigen; Carcinoma; DNA Methylation; DNA, Neoplasm; Female; Genes, APC; Humans; Kaplan-Meier Estimate; Lymphatic Metastasis; Male; Middle Aged; Promoter Regions, Genetic; Proportional Hazards Models; Stomach Neoplasms; Tumor Markers, Biological; Tumor Suppressor Proteins
DOI:
10.1016/j.mrfmmm.2015.05.002
