Τίτλος:
Heterogeneities in inflammatory and cytotoxic responses of RAW 264.7 macrophage cell line to urban air coarse, fine, and ultrafine particles from six European sampling campaigns
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
We investigated the cytotoxic and inflammatory activities of size-segregated particulate samples (particulate matter, PM) from contrasting air pollution situations in Europe. Coarse (PM10-2.5), fine (PM2.5-0.2), and ultrafine (PM0.2) particulate samples were collected with a modified Harvard high-volume cascade impactor (HVCI). Mouse RAW 264.7 macrophages were exposed to the samples for 24 h. Selected inflammatory mediators, nitric oxide (NO) and cytokines (tumor necrosis factor alpha [TNFα], interleukin 6 [IL-6], macrophage inflammatory protein-2 [MIP-2]), were measured together with cytotoxicity (MTT test), and analysis of apoptosis and cell cycle (propidium iodide staining). The PM10-2.5 samples had a much higher inflammatory activity than the PM2.5-0.2 and PM0.2 samples, but the PM2.5-0.2 samples showed the largest differences in inflammatory activity, and the PM0.2 samples in cytotoxicity, between the sampling campaigns. The PM2.5-0.2 samples from traffic environments in springtime Barcelona and summertime Athens had the highest inflammatory activities, which may be related to the high photochemical activity in the atmosphere during the sampling campaigns. The PM0.2 sample from wintertime Prague with proven impacts from local coal and biomass combustion had very high cytotoxic and apoptotic activities and caused a distinct cell cycle arrest. Thus, particulate size, sources, and atmospheric transformation processes affect the toxicity profile of urban air particulate matter. These factors may explain some of the heterogeneity observed in particulate exposure-response relationships of human health effects in epidemiological studies. Copyright © Informa Healthcare.
Συγγραφείς:
Jalava, P.I.
Salonen, R.O.
Pennanen, A.S.
Sillanpää, M.
Hälinen, A.I.
Happo, M.S.
Hillamo, R.
Brunekreef, B.
Katsouyanni, K.
Sunyer, J.
Hirvonen, M.-R.
Περιοδικό:
Inhalation Toxicology
Λέξεις-κλειδιά:
coal; cytokine; interleukin 6; macrophage inflammatory protein 2; nitric oxide; propidium iodide; tumor necrosis factor alpha, air particle control; air pollution; animal cell; apoptosis; article; atmosphere; biomass; cell cycle; cell cycle arrest; cell line; combustion; controlled study; cytotoxicity; exposure; genetic heterogeneity; inflammation; macrophage; mouse; nonhuman; particle size; particulate matter; photochemistry; priority journal; public health; staining; summer; urban area; winter, Air Pollutants; Animals; Cell Line; Cell Survival; Cytokines; Inflammation; Macrophages; Mice; Nitric Oxide; Particle Size; Particulate Matter
DOI:
10.1080/08958370601067863
