Τίτλος:
Effects of pulsed electric fields on DNA of human lymphocytes
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The effects of pulsed electric fields of low frequency (50 Hz) on DNA of human lymphocytes were investigated. The influence of additional external factors, such as hydrogen peroxide (H2O2) and γ-irradiation, as well as the repair efficiency in these lymphocytes, was also evaluated. The comet assay, a very sensitive and rapid method for detecting DNA damage at the single cells level was the method used. A significant amount of damage was observed after exposure to the electric fields, compared to the controls. After 2 h incubation at 37°C, a proportion of damage was repaired. H2O2 and γ-irradiation increased the damage to lymphocytes exposed to pulsed electric fields according to the dose used, while the amount of the repair was proportional to the damage. © 2006 Springer Science + Business Media, Inc.
Συγγραφείς:
Delimaris, J.
Tsilimigaki, S.
Messini-Nicolaki, N.
Ziros, E.
Piperakis, S.M.
Περιοδικό:
Cell Biology and Toxicology
Λέξεις-κλειδιά:
DNA; hydrogen peroxide, adult; article; comet assay; controlled study; DNA damage; DNA repair; exposure; gamma irradiation; genotoxicity; human; human cell; human experiment; incubation temperature; lymphocyte; male; priority journal; productivity; pulsed electric field, Adult; Comet Assay; DNA; DNA Damage; DNA Repair; Dose-Response Relationship, Radiation; Electromagnetic Fields; Gamma Rays; Humans; Hydrogen Peroxide; Lymphocytes; Male; Middle Aged; Oxidants
DOI:
10.1007/s10565-006-0105-1
