Τίτλος:
Development and validation of a reversed-phase ion-pair liquid chromatography method for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creams
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A reversed-phase ion-pair high-performance liquid chromatographic method was developed and validated for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creams. The determination was performed on a BDS C 18 analytical column (250 × 4.6 mm i.d., 5 μm particle size); the mobile phase consisted of 0.020 M tetrabutylammonium hydroxide and 0.025 M potassium dihydrogen phosphate (pH 6.8) mixed with acetonitrile in a ratio (77:23, v/v) and pumped at a flow rate 1.00 ml min-1. The UV detector was operated at 260 nm. The retention times of the magnesium ascorbyl phosphate, melatonin and chlorthalidone that was used as internal standard, were 6.55, 9.18 and 11.07 min, respectively. Calibration graphs are linear (r better than 0.9990, n = 6), in concentration range 1.00-10.00 μg ml-1 for magnesium ascorbyl phosphate and 0.63-6.25 μg ml-1 for melatonin. The intra- and inter-day R.S.D. values were less than 6.0%, while the relative percentage error Er was less than 3.5% (n = 5). The quantitation limits were 0.69 and 0.47 μg ml-1, for magnesium ascorbyl phosphate and melatonin, respectively. The method was applied to the analysis of a cosmetic cream and proved to be suitable for rapid and reliable quality control. © 2006 Elsevier B.V. All rights reserved.
Συγγραφείς:
Varvaresou, A.
Tsirivas, E.
Iakovou, K.
Gikas, E.
Papathomas, Z.
Vonaparti, A.
Panderi, I.
Περιοδικό:
Analytica Chimica Acta
Λέξεις-κλειδιά:
Concentration (process); Flow control; Ions; Magnesium compounds; Oxides; pH effects; Quality control, Cosmetics; Ion-pair liquid chromatography; Magnesium ascorbyl phosphate; Melatonin, High performance liquid chromatography, acetonitrile; ascorbic acid derivative; chlortalidone; cosmetic; magnesium ascorbyl phosphate; melatonin; potassium dihydrogen phosphate; tetrabutylammonium; unclassified drug, article; calibration; controlled study; cream; priority journal; quality control; quantitative analysis; reproducibility; reversed phase liquid chromatography; ultraviolet radiation; validation process
DOI:
10.1016/j.aca.2005.12.074
