Τίτλος:
Anticancer and biochemical effects of Viscum album L. protein extracts on HeLa cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
European mistletoe (Viscum album) is a medicinal plant with significant anticancer properties. In vitro callus production provides an essential alternative for biomass production and medicinal compounds propagation. In this study, we investigated (a) the biotechnological production of somaclonal proteins from mistletoe callus propagation and (b) the in vitro cytotoxic properties of these mistletoe protein extracts against cervical cancer. Mistletoe proteins (MPEs) were extracted from natural resources (plant leaves and stems) and in vitro propagated variant calluses. Potential cytotoxic effects of the extracted proteins on HeLa cells were assessed by the MTT proliferation assay, whereas oxidative damage and induction of apoptosis were measured fluorometrically by the reactive oxygen species (ROS) sensitive carboxy-H2-DCFDA method and caspase-3 activation assays, respectively. Electrophoretic results indicated differences in the protein bands between callus and donor plant tissues. Leaf and stem protein extracts (PEs) demonstrated a strong impact on HeLa cells viability by promoting oxidative damage and inducing apoptosis in contrast to callus PEs. This study shows that MPEs application in HeLa cells causes oxidative alterations and apoptosis through caspase-3 activation and increased intracellular ROS levels. These findings may be useful for uncovering novel bioactive products of pharmaceutical interest as well as for gaining an understanding of the biochemical pathways of mistletoe proteins in cancer treatment. © 2019, Springer Nature B.V.
Συγγραφείς:
Mavrikou, S.
Tsekouras, V.
Karageorgou, M.-A.
Moschopoulou, G.
Kintzios, S.
Περιοδικό:
Plant Cell, Tissue and Organ Culture
Λέξεις-κλειδιά:
Cell culture; Cell death; Chemical activation; Damage detection; Diseases; Plant extracts; Proteins, Anticancer properties; Biotechnological production; Caspase-3 activation; Hela cell line; Mistletoe extracts; Reactive oxygen species; Somaclonal variation; Therapeutic strategy, Lanthanum compounds, Callus; Cells; Diseases; Extractives; Lanthanum Compounds; Mistletoe; Production; Proteins
DOI:
10.1007/s11240-019-01733-0
